トルコのボトルの水の真菌汚染物質と真菌毒素を産生する可能性
Gülhan Tunç1, Fusun Uçar1, Osman Telli2
1Ege University, Department of Biology, Laboratory of Basic and Industrial Microbiology, Izmir, Turkey.
GMS hygiene and infection control
|August 29, 2025
まとめ
ペニシリウムやアスペルギルスのような真菌汚染物質がボトルの水で発見され,高濃度のミコトキシンシトリニンが検出されました. これは瓶詰め水の安全基準に 菌類の分析を含める必要性を強調しています
科学分野:
- 微生物学
- 食品の安全性
- 環境科学
背景:
- ボトルの水に関する現在の公衆衛生規制は 化学汚染物質と微生物汚染物質を扱っていますが 菌類汚染物質については 欠陥があります
- 商業用ボトルの水,特に19Lポリカーボネートボトルは広く消費されており,その微生物質の徹底的な評価が必要である.
研究 の 目的:
- アナトリア西部から供給され,イズミールおよび周辺の州で販売されている商業的なボトル水における真菌汚染物質の存在と種類を調査する.
- 菌類を特定し,ミコトキシン,特にシトリニンの生成の可能性を評価する.
主な方法:
- コロニー形成単位 (cfu) / 100 mlで表される数値で膜濾過を用いた真菌スクリーニング.
- ポリメラーゼ連鎖反応 (PCR) による内部転写スペーサー (ITS) 領域の増幅とシーケンシングによる真菌単離物の表型および遺伝子型識別.
- ミコトキシン形成の可能性のスクリーニングは,アンモニア蒸気試験,液体染色体質量測定 (LC-MS) および高性能液体染色体測定 (HPLC) を使用します.
主要な成果:
- 主要な菌株はクラドスポリウム,ペニシリウム,アルターナリアでした.
- ミコトキシンシトリニンの高濃度 (411.586μg/l) が検出され,飲料の基準を超えました.
- 分子識別はアクチン遺伝子領域によってサポートされ,種割り当てが確認されました.
結論:
- 菌類の汚染物質とミコトキシンが瓶詰め水に含まれる大きなリスクであり,水分析基準に組み込まれなければならない.
- この研究は,トルコで初めて瓶詰め水における真菌汚染とミコトキシンの存在に関する詳細な研究です.
- この結果は,消費者の安全を確保するために,ボトル詰め水の品質に対する規制の強化の重要性を強調しています.
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