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Updated: Sep 9, 2025

PCR Mutagenesis, Cloning, Expression, Fast Protein Purification Protocols and Crystallization of the Wild Type and Mutant Forms of Tryptophan Synthase
09:31

PCR Mutagenesis, Cloning, Expression, Fast Protein Purification Protocols and Crystallization of the Wild Type and Mutant Forms of Tryptophan Synthase

Published on: September 26, 2020

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浮気性フラビン依存酵素によるペプチドトリプトファンハロゲン化

Andrew J Rice1,2, Mayuresh G Gadgil2, Paola Bisignano3

  • 1Department of Biochemistry, Vanderbilt University School of Medicine-Basic Science, Department of Chemistry, Vanderbilt University, Nashville, TN, 37232, USA.

Angewandte Chemie (International ed. in English)
|August 30, 2025
PubMed
まとめ

研究 者 たち は,ペプチド の 内側 の アミノ酸 を 変化 さ せる 汎用 な 酵素,ChlH (フラビン 依存 ハロゲナーゼ) を 特定 し まし た. この発見により,生物技術における酵素ペプチド合成と改変の可能性が広がります.

キーワード:
生物触媒塩素ハロゲン化ペプチドタンパク質

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