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Updated: May 6, 2026

Metabolic Labeling and Membrane Fractionation for Comparative Proteomic Analysis of Arabidopsis thaliana Suspension Cell Cultures
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細胞系,異種移植,患者の腫瘍の質量スペクトロメトリのための超遠心分離によるプラズマ膜濃縮のためのプロトコル

Amber K Hamilton1, Brian Mooney2, Tina Glisovic-Aplenc1

  • 1Center for Childhood Cancer Research and Division of Oncology, Children's Hospital of Philadelphia, Philadelphia, PA 19104, USA.

STAR protocols
|August 30, 2025
PubMed
まとめ

この研究は,免疫療法研究のために,がん細胞から血タンパク質を分離するための新しいプロトコルを導入しています. この方法は,薬の開発のための潜在的な癌標的を特定するのに役立ちます.

キーワード:
癌について細胞生物学細胞膜臨床プロトコル免疫学マススペクトロメトリータンパク質の生化学プロテオミクス構造生物学

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Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.
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