Jove
Visualize
お問い合わせ
JoVE
x logofacebook logolinkedin logoyoutube logo
JoVEについて
概要リーダーシップブログJoVEヘルプセンター
著者向け
出版プロセス編集委員会範囲と方針査読よくある質問投稿
図書館員向け
推薦の声購読アクセスリソース図書館諮問委員会よくある質問
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experimentsアーカイブ
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教員リソースセンター教員サイト
利用規約
プライバシーポリシー
ポリシー

関連する概念動画

Nuclear Export01:42

Nuclear Export

3.7K
The nucleus restricts several proteins within and allows others to pass. The restricted proteins possess a nuclear retention sequence or NRS, anchoring them to the nuclear lamins and preventing their transport to the cytosol. The non-restricted proteins, after their synthesis, are transported to their site of action, such as the cytosol or other organelles, with the help of nuclear export signals or NES.
NES are of three types- the canonical 10-residue long leucine-rich signal and other...
3.7K
Nuclear Protein Sorting01:34

Nuclear Protein Sorting

4.7K
Nuclear protein sorting is the selective trafficking of histones, polymerases, gene regulatory proteins into the nucleus and exporting RNAs and ribosomes to the cytosol. It is a tightly controlled process that regulates gene expression within a cell.
Proteins targeted to the nucleus carry nuclear localization signals or NLS recognized by import receptors in the cytosol. Similarly, proteins with nuclear export signals are recognized by export receptors. Import and export receptors are...
4.7K
Post-translational Translocation of Proteins to the RER01:27

Post-translational Translocation of Proteins to the RER

5.9K
A sizable fraction of proteins destined for ER are first synthesized in the cell cytosol and then transported across the ER membrane–a process called post-translational translocation. Similar to cotranslationally translocated proteins, these proteins also use the Sec translocon complex to enter the ER lumen.
Targeting proteins to the ER
Hsp40 and Hsp70 chaperone molecules bind the translated proteins in the cytosol to prevent their folding. The chaperone binding helps to keep the signal...
5.9K
Cotranslational Protein Translocation01:20

Cotranslational Protein Translocation

7.6K
Translocation of proteins across membranes is an ancient process that occurs even in bacteria and archaebacteria. In fact, the components of the translocation machinery are still conserved between prokaryotes and eukaryotes.
Sec61 channel partners for cotranslational translocation
During cotranslational translocation, the Sec61 channel partners with the signal recognition particle (SRP), the signal recognition particle receptor (SR), and the ribosomes to transport the nascent polypeptide chain...
7.6K
Translocation of Proteins into the Mitochondria01:19

Translocation of Proteins into the Mitochondria

3.2K
Mitochondrial precursors are translocated to the internal subcompartments via independent mechanisms involving distinct protein machineries called translocases.
Sorting of outer membrane proteins:
Mitochondrial outer membrane proteins are of two types: the transmembrane, beta-barrel porins, and the membrane-anchored, alpha-helical proteins. Beta-barrel porin precursors are translocated by the TOM complex and inserted into the outer mitochondrial membrane by the SAM complex. In contrast,...
3.2K
Protein Translocation Machinery on the ER Membrane01:28

Protein Translocation Machinery on the ER Membrane

4.9K
The translocon complex situated on the ER membrane is the main gateway for the protein secretory pathway. It facilitates the transport of nascent peptides into the ER lumen and their insertion into the ER membrane.
Sec61 protein conducting channel
In eukaryotes, the translocon complex comprises a core heterotrimeric translocator channel called the Sec61 complex. This channel includes three transmembrane proteins, Sec61α, Sec61β, and Sec61γ, and is the largest subunit of the...
4.9K

こちらも読む

関連記事

共著者、ジャーナル、引用グラフによってこの研究に関連する記事。

並び替え
Same author

Safety and Feasibility of Transcaval Access for the Delivery of Impella Microaxial Flow Pump.

Journal of the Society for Cardiovascular Angiography & Interventions·2025
Same author

Utility of High-Sensitivity Cardiac Troponin-T and N-Terminal Pro-B-Type Natriuretic Peptide to Predict Survival, Quality of Life, and Functional Status Changes After Transcatheter Aortic Valve Implantation.

Journal of the American Heart Association·2025
Same author

Student leadership in planetary health: insights from the planetary health report card leadership team.

BMJ leader·2025
Same author

Management of extracervical recurrence following total parathyroidectomy and autotransplantation for renal hyperparathyroidism.

Scandinavian journal of surgery : SJS : official organ for the Finnish Surgical Society and the Scandinavian Surgical Society·2025
Same author

Therapies and Pipeline for Hepatitis B and Hepatitis D.

Clinics in liver disease·2025
Same author

Artificial Intelligence and Machine Learning Applications in Liver Disease.

Clinics in liver disease·2025

関連する実験動画

Updated: Sep 9, 2025

Purification of the Membrane Compartment for Endoplasmic Reticulum-associated Degradation of Exogenous Antigens in Cross-presentation
12:48

Purification of the Membrane Compartment for Endoplasmic Reticulum-associated Degradation of Exogenous Antigens in Cross-presentation

Published on: August 21, 2017

8.3K

核酸に依存する形状の変化 輸送機による直接ペプチドの輸出

James Lee1, Victor Manon2, Jue Chen3

  • 1Laboratory of Membrane Biophysics and Biology, the Rockefeller University, New York, NY 10065, USA.

Immunity
|August 30, 2025
PubMed
まとめ

抗原処理に関連するトランスポーター (TAP) は,免疫反応のためにペプチドをエンドプラズマの網膜に移動させます. TAPがペプチドを輸送し放出し ERのストレスを防ぐ方法を明らかにします

キーワード:
ABCトランスポーターMHC-I適応免疫抗原の表示ヌクレオチド結合領域抗原処理に関連するトランスポーター

さらに関連する動画

Antimicrobial Peptides Produced by Selective Pressure Incorporation of Non-canonical Amino Acids
11:56

Antimicrobial Peptides Produced by Selective Pressure Incorporation of Non-canonical Amino Acids

Published on: May 4, 2018

12.6K
Production of Disulfide-stabilized Transmembrane Peptide Complexes for Structural Studies
12:05

Production of Disulfide-stabilized Transmembrane Peptide Complexes for Structural Studies

Published on: March 6, 2013

14.3K

関連する実験動画

Last Updated: Sep 9, 2025

Purification of the Membrane Compartment for Endoplasmic Reticulum-associated Degradation of Exogenous Antigens in Cross-presentation
12:48

Purification of the Membrane Compartment for Endoplasmic Reticulum-associated Degradation of Exogenous Antigens in Cross-presentation

Published on: August 21, 2017

8.3K
Antimicrobial Peptides Produced by Selective Pressure Incorporation of Non-canonical Amino Acids
11:56

Antimicrobial Peptides Produced by Selective Pressure Incorporation of Non-canonical Amino Acids

Published on: May 4, 2018

12.6K
Production of Disulfide-stabilized Transmembrane Peptide Complexes for Structural Studies
12:05

Production of Disulfide-stabilized Transmembrane Peptide Complexes for Structural Studies

Published on: March 6, 2013

14.3K

科学分野:

  • 分子生物学
  • 免疫学
  • 構造生物学

背景:

  • 抗原処理 (TAP) に関連するトランスポーターは,適応性免疫に不可欠です.
  • TAPは,抗原性ペプチドの細胞質から内 плазма網膜 (ER) への輸送を容易にする.
  • このプロセスは,ペプチドをメジャー・ヒストコンパティビリティ・コンプレックスクラスI (MHC-I) の分子にロードするために不可欠です.

研究 の 目的:

  • 人間の TAP ヘテロダイマーによるペプチド輸送と放出の構造的メカニズムを解明する.
  • TAPの運送サイクル全体における機能的状態を理解する.
  • 構造的な基盤を提供するために ヴァンダート・トラッピングとトランス・インヒビションのような現象.

主な方法:

  • 高解像度構造を決定するために,冷凍電子顕微鏡 (cryo-EM) が使用されました.
  • ヒトのTAPヘテロダイマーの構造は複数の機能状態で捉えられた.
  • 分析はATP結合と水解に関連する構造変化に焦点を当てた.

主要な成果:

  • TAPの明確な内向きと外向きの形状が解消されました.
  • ATP結合は内向きの状態を安定させ,ペプチド腔を細胞溶液に露出させる.
  • 外向きの状態への移行は,ER ルーメナル放出のためのペプチド結合部位を再構成する.
  • ATPの水解と核酸結合ドメインの分離が輸送サイクルを駆動し,トランスポーターをリセットします.

結論:

  • TAPによるペプチド輸送のための包括的な構造的枠組みが確立されています.
  • この発見はペプチドの一方的な輸送と放出のメカニズムを説明する.
  • この研究は,ERストレスを防止するフィードバックメカニズムであるトランス抑制の構造的基礎を明らかにします.