関連する実験動画

Updated: Apr 14, 2026

Cultivation of Mammalian Cells Using a Single-use Pneumatic Bioreactor System
11:42

Cultivation of Mammalian Cells Using a Single-use Pneumatic Bioreactor System

Published on: October 9, 2014

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カプリアビドスネキャターH16によるSCP生産のためのパイロットスケールの圧力ガスバイオリアクターの性能

Kay Domenico Novak1, Petra Heidinger1, Daniel Schwendenwein1

  • 1acib GmbH, Krenngasse 37, Graz A-8010, Austria.

Journal of biotechnology
|August 30, 2025
PubMed
まとめ

キモリトオートロフィック・インノキュラムは,Cupriavidus necator H16を用いて単細胞タンパク質 (SCP) の生産効率を大幅に高める. この方法は遅延段階を回避し,持続可能な飼料の代替品のためにより速く,スケーラブルで高品質のタンパク質の収穫を可能にします.

キーワード:
ケモリトオートロフィックカプリアヴィドゥス・ネカターガス発酵細胞密度が高いパイロットプラント単細胞タンパク質

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Bioreactor Design and Operational System01:29

Bioreactor Design and Operational System

Bioreactors are engineered vessels designed to cultivate microorganisms under controlled conditions for industrial bioprocessing. They maintain sterility and allow precise regulation of pH, temperature, oxygen, and nutrient levels to optimize microbial growth and metabolite production. Bioreactors range from small laboratory units of 1 liter to industrial systems holding up to 500,000 liters, though only about 75% of their volume is actively used for fermentation. The remaining headspace...
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Bioreactor Controls-II01:18

Bioreactor Controls-II

In aerobic fermentations, oxygen is vital for microbial growth and metabolite production. Since air comprises only about 20% oxygen and the gas is poorly soluble in water—just 9 ppm at 20°C—supplying sufficient oxygen becomes a critical challenge, especially in high-demand processes like yeast growth or citric acid production. Even a fully saturated broth may offer only a few seconds of oxygen availability.To address this, sterile or scrubbed air is introduced into the...
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Designing Growth Media for Bioreactors01:30

Designing Growth Media for Bioreactors

Growth media provide essential nutrients that support cell growth and metabolism, thereby enhancing the yield of valuable products such as enzymes, antibiotics, and biomass. Designing an effective growth medium involves balancing all components to prevent nutrient limitations or toxic excesses, both of which can impair growth and reduce product yields.Composition of a Typical Growth MediumA typical growth medium contains carbon and nitrogen sources, salts, vitamins, trace elements, and...
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Scale-Up Processes01:14

Scale-Up Processes

The scale-up of microbial fermentation processes is essential in industrial biotechnology, allowing the transition from laboratory-scale experiments to commercial-scale production while aiming to maintain product yield and quality. This process requires meticulous adjustment of equipment design, process parameters, and contamination control strategies to accommodate increasing culture volumes.At the laboratory scale, cultures are typically maintained in 1 to 10-liter glass or autoclavable...
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Upstream Processing01:27

Upstream Processing

Upstream processing represents a critical phase in biomanufacturing, wherein biological systems such as microorganisms, mammalian cells, or insect cells are cultivated to produce therapeutic proteins, vaccines, enzymes, or other biologically derived products. This phase encompasses all steps from the selection and genetic manipulation of the production organism to the cultivation of cells in bioreactors under tightly controlled environmental conditions.Host Selection and Genetic OptimizationThe...
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