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Updated: Sep 9, 2025

Simple and Fast Rolling Circle Amplification-Based Detection of Topoisomerase 1 Activity in Crude Biological Samples
10:13

Simple and Fast Rolling Circle Amplification-Based Detection of Topoisomerase 1 Activity in Crude Biological Samples

Published on: December 2, 2022

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非対称なTセグメント結合とゲートダイナミクスは,IIA型トポイソメラーゼの触媒サイクルの最終段階を支配する

Kristina Stevanović1, Barbara Herlah2, Matic Pavlin3

  • 1Theory Department, National Institute of Chemistry, Hajdrihova 19, 1000 Ljubljana, Slovenia; Laboratory of Bioinformatics and Computational Chemistry, Institute of Nuclear Sciences Vinca, Serbia.

International journal of biological macromolecules
|August 31, 2025
PubMed
まとめ

IIA型DNAトポイソメラーゼと呼ばれる 分子ナノマシンは 協調ゲートダイナミクスを通して DNA処理を完了します Cゲート内の核酸結合とDNAセグメントの位置づけは,酵素活性とDNA放出を調節する.

キーワード:
Cゲート触媒サイクル分子動力学TセグメントタイプIIADNAトポイソメラーゼ

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DNA Topoisomerases02:02

DNA Topoisomerases

Topoisomerases are enzymes that relax overwound DNA molecules during various cell processes, including DNA replication and transcription. These enzymes regulate positive and negative DNA supercoiling without changing the nucleotide sequence. DNA overwinding in a clockwise direction results in positively supercoiled DNA, whereas underwinding in a counterclockwise direction produces negatively supercoiled DNA.
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DNA Helicases

DNA unwinding helicase enzymes are a type of motor protein. Motor proteins can translocate along filaments or polymers using energy generated from ATP hydrolysis. Helicases are involved in all the important cellular processes where DNA unwinding is required, such as DNA replication, repair, recombination, and transcription. They are present in all living organisms, but vary in their structure, function, and mechanism of action. For example, in prokaryotes, DnaB helicase binds and translocates...
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Actin Polymerization

Actin polymerization occurs through the head-to-tail association of binding sites on monomeric actin or G-actin to form filamentous or F-actin. The polymerization can be divided into three phases ̶  nucleation, elongation, and steady-state phase.
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Translesion DNA Polymerases

Translesion (TLS) polymerases rescue stalled DNA polymerases at sites of damaged bases by replacing the replicative polymerase and installing a nucleotide across the damaged site. Doing so, TLS allows additional time for the cell to repair the damage before resuming regular DNA replication.
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