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Updated: Sep 9, 2025

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N末端アセチルトランスフェラーゼDの浄化と活性測定

Yi-Hsun Ho1, Emma K Seipp1, Rong Huang1

  • 1Borch Department of Medicinal Chemistry and Molecular Pharmacology, Purdue Institute for Drug Discovery, Purdue University Center for Cancer Research, Purdue University, West Lafayette, IN, United States.

Methods in enzymology
|August 31, 2025
PubMed
まとめ

アルファ-N-末端アセチルトランスフェラーゼD (NatD) の活性を測定する2つの測定法を開発しました. これらの方法は,遺伝子調節の研究と,NatD媒介によるアセチル化のための新しい阻害剤の発見に不可欠です.

キーワード:
MALDI-MS についてNα-アセチル化アセチル化アセチルトランスフェラーゼ光測定法

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Phase II Reactions: Acetylation Reactions01:24

Phase II Reactions: Acetylation Reactions

Acetylation, a phase II biotransformation reaction, introduces an acetyl group to drugs or their metabolites. Acetyltransferase enzymes facilitate this reaction, which resembles α-amino acid conjugation due to the addition of a functional group to the drug molecule.
The substrates for acetylation are typically drugs or their metabolites with an amino, sulfonamide, or hydrazine functional group. Acetylation can occur at several points in the drug molecule, including primary, secondary, and...
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