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関連する概念動画

Overview of Electron Microscopy01:25

Overview of Electron Microscopy

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The wavelengths of visible light ultimately limit the maximum theoretical resolution of images created by light microscopes. Most light microscopes can only magnify 1000X, and a few can magnify up to 1500X. Electrons, like electromagnetic radiation, can behave like waves, but with wavelengths of 0.005 nm, they produce significantly greater resolution up to 0.05 nm as compared to 500 nm for visible light. An electron microscope (EM) can create a sharp image that is magnified up to 2,000,000X.
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Transmission Electron Microscopy01:15

Transmission Electron Microscopy

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In 1931, physicist Ernst Ruska—building on the idea that magnetic fields can direct an electron beam just as lenses can direct a beam of light in an optical microscope—developed the first prototype of the electron microscope. This development led to the development of the field of electron microscopy. In the transmission electron microscope (TEM), electrons are produced by a hot tungsten element and accelerated by a potential difference in an electron gun, which gives them up to 400...
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Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

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Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
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Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
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Three-Dimensional Microscopy in Microbiology01:28

Three-Dimensional Microscopy in Microbiology

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Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...
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Scanning Electron Microscopy01:07

Scanning Electron Microscopy

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A scanning electron microscope (SEM) is used to study the surface features of a sample by using an electron beam that scans the sample surface in a two-dimensional manner. Typically, areas between ~1 centimeter to 5 micrometers in width can be imaged. SEM can be used to image bacteria, viruses, tissues as well as larger samples like insects. Conventional SEM gives a magnification ranging from 20X to 30,000X and spatial resolution of 50 to 100 nanometers.
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Updated: Sep 9, 2025

Characterization Of Multi-layered Fish Scales Atractosteus spatula Using Nanoindentation, X-ray CT, FTIR, and SEM
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高解像度伝送電子顕微鏡を用いたデントンの多尺度構造を明らかにする

M Leclercq1, M Vallet1,2, T Reiss1

  • 1Université Paris-Saclay, CentraleSupélec, ENS Paris-Saclay, CNRS, LMPS - Laboratoire de Mécanique Paris-Saclay, Gif-sur-Yvette, France.

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まとめ
この要約は機械生成です。

この研究は,高解像度TEMを用いて,歯質内のコラーゲン線維とヒドロキシアパタイト結晶のナノスケール組織を明らかにしています. 歯皮 の 詳細 を 明らか に する

キーワード:
繊維のコラーゲンハイドロキシアパタイトナノ構造構造と性質の関係歯の部品トロポコラーゲン

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Miniaturized Sample Preparation for Transmission Electron Microscopy
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Preparation and Observation of Thick Biological Samples by Scanning Transmission Electron Tomography
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関連する実験動画

Last Updated: Sep 9, 2025

Characterization Of Multi-layered Fish Scales Atractosteus spatula Using Nanoindentation, X-ray CT, FTIR, and SEM
10:06

Characterization Of Multi-layered Fish Scales Atractosteus spatula Using Nanoindentation, X-ray CT, FTIR, and SEM

Published on: July 10, 2014

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Miniaturized Sample Preparation for Transmission Electron Microscopy
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Published on: July 27, 2018

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Preparation and Observation of Thick Biological Samples by Scanning Transmission Electron Tomography
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Preparation and Observation of Thick Biological Samples by Scanning Transmission Electron Tomography

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科学分野:

  • バイオマテリアル科学
  • ナノテクノロジー
  • 歯科 研究

背景:

  • デントンの微細構造は2D顕微鏡でよく理解されています.
  • 3Dの微細構造分析により 歯の多孔ネットワークが明らかになりました
  • コラーゲン繊維 (CF) と歯質中の鉱物結晶のナノスケール組織は不明である.

研究 の 目的:

  • コラーゲン・フィブリルとミネラル・クリスタル組織を中心に,ナノスケールでデンチンナノ構造を分析する.
  • デンチン-エナメル接合点 (DEJ) の近くと,中央のデンチンを調査する.
  • 鉱物/有機の絡み合いを調査するためのTEMサンプル収集のプロトコルを提案する.

主な方法:

  • 高解像度伝送電子顕微鏡 (TEM)
  • 選択された領域の電子 difraktion.
  • トゥーブル軸に対するTEM断面の方向

主要な成果:

  • CFsとHAP鉱物の織り込みが明らかになった.
  • 観察されたHAP結晶は,結晶軸に沿って伸び,CFの周りにS形構造を形成した.
  • トゥーブル軸に平行なCFを特定し,裂け目の拡散を潜在的に説明します.

結論:

  • この研究では 歯質のナノスケール構造が解明され 鉱物と有機が絡み合っていることが明らかになりました
  • この発見は,構造的グラデーションと歯茎の構造特性の関係に洞察を与えます.
  • 提案されたTEMプロトコルは,デンチンナノ構造のさらなる調査を容易にする.