血球腫由来幹細胞の増殖,血管新生およびアディポゲネシスに対するノッチ信号の効果

Weidong Wang¹, Sheng Chen¹, Yuan Wang¹

¹Department of Burns and Plastic Surgery, Children's Hospital of Nanjing Medical University, Nanjing, Jiangsu.

European journal of histochemistry : EJH

|September 1, 2025

まとめ

血球腫幹細胞におけるノッチシグナル伝達抑制は,PI3K/ AKTの活性を下げることで増殖とアディポゲネシスを促進する. この発見は幼児の血管腫の逆行メカニズムに新たな洞察をもたらします

科学分野:

幹細胞生物学
分子信号
発達生物学

背景:

乳児性血管腫 (IH) の回復の鍵となるのは,血管腫由来幹細胞 (Hem- SCs) です.
ハッチ・シグナリングは,Hem-SCsの脂肪形成に関与している.

研究の目的:

ヘム-SCにおけるノッチ・シグナル伝達の役割を調査する.
ヘム-SCsの増殖,血管新生,およびアディポゲネシスに対するノッチ抑制の効果を決定する.

主な方法:

IH標本から分離されたプライマリ Hem-SCs.
γ- 分泌酵素阻害剤 DAPTによる治療
ノッチ,PI3K/AKTシグナル伝達,VEGFR発現,増殖,アディポゲンマーカーの評価

主要な成果:

DAPTはノッチとPI3K/AKTのシグナリングを抑制し,増殖と血管新生を強めた.
DAPTはVEGFR1とVEGFR2の発現を差異的に調節した.
アディポゲンマーカーと脂質蓄積の増加と相関する.
PI3Kの活性化により,DAPT誘発のアディポゲネシスが逆転した.

結論:

ヘム-SCsの増殖,血管新生,脂肪細胞の分化を促進する.
この効果はPI3K/AKTシグナル伝達の弱化によって媒介される.
ターゲティング・ノッチ・シグナリングは,IHの治療戦略を提供することができる.

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