Jove
Visualize
お問い合わせ
JoVE
x logofacebook logolinkedin logoyoutube logo
JoVEについて
概要リーダーシップブログJoVEヘルプセンター
著者向け
出版プロセス編集委員会範囲と方針査読よくある質問投稿
図書館員向け
推薦の声購読アクセスリソース図書館諮問委員会よくある質問
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experimentsアーカイブ
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教員リソースセンター教員サイト
利用規約
プライバシーポリシー
ポリシー

関連する概念動画

Cell Migration01:09

Cell Migration

17.2K
Cell migration, the process by which cells move from one location to another, is essential for the proper development and viability of organisms throughout their life. When cells are not able to migrate properly to their ordained locations, various disorders may occur. For example, disruption in cell migration causes chronic inflammatory diseases such as arthritis.
17.2K
Immune Surveillance by NK Cells and Phagocytes01:25

Immune Surveillance by NK Cells and Phagocytes

2.7K
Immune surveillance is an integral part of the innate immune system, involving the continuous monitoring of peripheral tissues to detect and respond to pathogens, infected cells, or cancerous cells. This surveillance is conducted primarily by natural killer (NK) cells and phagocytes, which employ distinct but complementary mechanisms to identify and eliminate threats.
Natural Killer Cells: The Fast Responders
NK cells are large granular lymphocytes found in the blood and lymphatic system. These...
2.7K
Phagocytosis00:41

Phagocytosis

6.6K
Cells pull particles inward and engulf them in spherical vesicles in an energy-requiring process called endocytosis. Phagocytosis ("cellular eating") is one of three major types of endocytosis. Cells use phagocytosis to take in large objects, such as other cells (or their debris), bacteria, and even viruses.
The objective of phagocytosis is often destruction. Cells use phagocytosis to eliminate unwelcome visitors, like pathogens (e.g., viruses and bacteria). Many immune system cells,...
6.6K
Chemotaxis and Direction of Cell Migration01:21

Chemotaxis and Direction of Cell Migration

3.5K
Cells can detect chemical cues in their environment and reorganize the cytoskeleton to migrate toward them or away from them. This directional migration, called chemotaxis, is essential during embryogenesis and development, immune response, tissue repair and regeneration, and reproduction. These chemical cues can either attract or repel the cell's movement. For example, axon development is determined by a combination of chemoattractants and chemorepellents that direct the growing axon...
3.5K
Phagocytosis of Apoptotic Cells01:17

Phagocytosis of Apoptotic Cells

4.0K
Cells undergoing apoptosis form apoptotic bodies that must be removed immediately to prevent inflammation, autoimmune diseases, and necrosis. Phagocytosis is carried out by professional phagocytes such as macrophages or  immature dendritic cells. Non-professional phagocytes such as  epithelial cells and fibroblasts also take part in this process; however, they are not as effective as professional phagocytes. 
Normal cells contain receptors that prevent them from being recognized...
4.0K
Cell-mediated Immune Responses01:40

Cell-mediated Immune Responses

72.2K
Overview
72.2K

こちらも読む

関連記事

共著者、ジャーナル、引用グラフによってこの研究に関連する記事。

並び替え
Same author

Low-Level Domoic Acid Exposure Induces Age-like Cardiomyopathy in Young Adult and Aged Mice.

Marine drugs·2026
Same author

Peptoid-Based Nanosheets Exhibiting Broad Antiviral Activity against Enveloped RNA Viruses.

ACS nano·2026
Same author

The Arp2/3 complex is required for in situ haptotactic response of microglia to iC3b.

EMBO reports·2026
Same author

Local antibody feedback enforces a checkpoint on affinity maturation in the germinal center and promotes epitope spreading.

Immunity·2026
Same author

Insights into the structural differences between homo- and heterodimers enriched from a cocktail of monoclonal antibodies against SARS-CoV-2.

Scientific reports·2026
Same author

On the Feasibility of Clinical Studies with Cross-Linking Mass Spectrometry.

Journal of proteome research·2026

関連する実験動画

Updated: Sep 9, 2025

"Phagosome Closure Assay" to Visualize Phagosome Formation in Three Dimensions Using Total Internal Reflection Fluorescent Microscopy TIRFM
10:07

"Phagosome Closure Assay" to Visualize Phagosome Formation in Three Dimensions Using Total Internal Reflection Fluorescent Microscopy TIRFM

Published on: August 26, 2016

6.5K

物理的な閉じ込めとファゴサイトの吸収は,持続的な細胞移動を誘導する.

Summer G Paulson1,2, Sophia Liu1,2, Jeremy D Rotty1

  • 1Uniformed Services University of the Health Sciences, Department of Biochemistry, Bethesda, MD, 20814, USA.

Biology open
|September 1, 2025
PubMed
まとめ

物理的な閉じ込めは,BV2マイクログリアのような細胞のファゴシトーシスを著しく強化し,強力な駆動力として作用します. この効果にはArp2/3複合体とミオシンIIが関与し,細胞の移動と免疫反応に影響する.

キーワード:
アクチンArp2/3 コンプレックス限られた運動能力フィブロネクチンミクログリアミオシンファゴサイトーシス

さらに関連する動画

Study of Cell Migration in Microfabricated Channels
09:36

Study of Cell Migration in Microfabricated Channels

Published on: February 21, 2014

12.1K
Time-lapse 3D Imaging of Phagocytosis by Mouse Macrophages
07:24

Time-lapse 3D Imaging of Phagocytosis by Mouse Macrophages

Published on: October 19, 2018

14.7K

関連する実験動画

Last Updated: Sep 9, 2025

"Phagosome Closure Assay" to Visualize Phagosome Formation in Three Dimensions Using Total Internal Reflection Fluorescent Microscopy TIRFM
10:07

"Phagosome Closure Assay" to Visualize Phagosome Formation in Three Dimensions Using Total Internal Reflection Fluorescent Microscopy TIRFM

Published on: August 26, 2016

6.5K
Study of Cell Migration in Microfabricated Channels
09:36

Study of Cell Migration in Microfabricated Channels

Published on: February 21, 2014

12.1K
Time-lapse 3D Imaging of Phagocytosis by Mouse Macrophages
07:24

Time-lapse 3D Imaging of Phagocytosis by Mouse Macrophages

Published on: October 19, 2018

14.7K

科学分野:

  • 細胞生物学
  • 免疫学
  • バイオ物理学

背景:

  • ファゴシトーシスは通常,非限定の in vitro 設定で研究されます.
  • 封じ込め状態がファゴサイトーシスに及ぼす影響はよくわかっていない.
  • ミクログリアは先天的な免疫反応に 重要な役割を果たします

研究 の 目的:

  • BV2マイクログリアのような細胞におけるIgG媒介のファゴシトーシスに対する身体的拘束の影響を調査する.
  • 閉じ込めモジュールされたファゴサイトーシスと細胞移動の基礎となる細胞骨格のメカニズムを解明する.
  • 封じ込めと細胞粘着への依存を調査する.

主な方法:

  • 閉じ込められたおよび閉じ込められていない in vitro アッセイの両方で BV2 マイクログリアのような細胞を使用した.
  • 光珠を用いたIgG媒介のファゴサイトーシスの研究.
  • Arp2 / 3複合体,ミオシンII,およびインテグリン依存粘着の作用を薬理学的阻害剤と遺伝的障害を用いて調べました.
  • 生細胞画像を用いた細胞の移動と"食細胞原始化"を評価した.

主要な成果:

  • 物理的な閉じ込めは,閉じ込められていない状態と比較して,ファゴサイト細胞の割合を大幅に増加させた.
  • 閉塞は,ミオシンIIの破壊時にファゴシトの吸収を部分的に回復させ,シトカラーシンDに対する部分的な抵抗をもたらした.
  • リンゴの吸収は,インテグリン依存性の付着を必要とする"ファゴシット・プライミング"と呼ばれる持続的な細胞移動を刺激した.
  • 包囲された環境 (ミオシンII,Arp2/3複合体が必要) と包囲されていない環境の間では,ファゴシタプリミングのための細胞骨格の要求は異なっていた.

結論:

  • 身体的拘束はマイクログリアの 細胞化を強力に促進します
  • 封じ込めは,ファゴシトーシスとファゴシトプリミングの細胞骨格のダイナミクスを変化させる.
  • 封じ込めによって調節されるファゴシートプリミングは,傷の監視のための重要な先天的な免疫メカニズムである可能性があります.