Jove
Visualize
お問い合わせ
JoVE
x logofacebook logolinkedin logoyoutube logo
JoVEについて
概要リーダーシップブログJoVEヘルプセンター
著者向け
出版プロセス編集委員会範囲と方針査読よくある質問投稿
図書館員向け
推薦の声購読アクセスリソース図書館諮問委員会よくある質問
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experimentsアーカイブ
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教員リソースセンター教員サイト
利用規約
プライバシーポリシー
ポリシー

関連する概念動画

Comparing Copy Number Variations and SNPs02:26

Comparing Copy Number Variations and SNPs

Sequencing of the human genome has opened up several best-kept secrets of the genome. Scientists have identified thousands of genome variations that exist within a population. These variations can be a single nucleotide or a larger chromosomal variation.
Copy number variations or CNVs are the structural variations that cover more than 1kb of DNA sequence. The single nucleotide polymorphism (SNP), on the other hand, is a single nucleotide change or a point mutation that is found in more than 1%...
Distributions to Estimate Population Parameter01:26

Distributions to Estimate Population Parameter

The accurate values of population parameters such as population proportion, population mean, and population standard deviation (or variance) are usually unknown. These are fixed values that can only be estimated from the data collected from the samples. The estimates of each of these parameters are sample proportion, the sample mean, and sample standard deviation (or variance). To obtain the values of these sample statistics, data are required that have particular distribution and central...
Distribution Reliability and Automation01:25

Distribution Reliability and Automation

Distribution reliability in electrical power systems is critical for ensuring an uninterrupted power supply to consumers at minimal cost. According to IEEE Standard Terms, reliability is the probability that a device will function without failure over a specified time period or amount of usage. For electric power distribution, this translates to maintaining continuous power supply and addressing customer concerns over power outages. Several indices, as defined by IEEE Standard 1366-2012, are...
One-Compartment Open Model: Wagner-Nelson and Loo Riegelman Method for ka Estimation01:24

One-Compartment Open Model: Wagner-Nelson and Loo Riegelman Method for ka Estimation

This lesson introduces two critical methods in pharmacokinetics, the Wagner-Nelson and Loo-Riegelman methods, used for estimating the absorption rate constant (ka) for drugs administered via non-intravenous routes. The Wagner-Nelson method relates ka to the plasma concentration derived from the slope of a semilog percent unabsorbed time plot. However, it is limited to drugs with one-compartment kinetics and can be impacted by factors like gastrointestinal motility or enzymatic degradation.
On...
Statistical Software for Data Analysis and Clinical Trials01:12

Statistical Software for Data Analysis and Clinical Trials

Statistical software is pivotal in data analysis and clinical trials by providing tools to analyze data, draw conclusions, and make predictions. These software packages range from simple data management applications to complex analytical platforms, supporting various statistical tests, models, and simulation techniques. Their significance lies in their ability to handle vast amounts of data with precision and efficiency, enabling researchers to validate hypotheses, identify trends, and make...
Genetic Variation01:25

Genetic Variation

Genetic variation is the diversity in DNA sequences found among individuals of the same species. This diversity is crucial for a species' survival because it helps organisms adapt to environmental changes. Genetic variation begins with fertilization, where an egg and sperm cell merge. Each of these cells carries 23 chromosomes, up to 46 in the fertilized egg. Chromosomes are long DNA strands that contain genes, the basic units of heredity.
Genes exist in different versions called alleles, which...

こちらも読む

関連記事

共著者、ジャーナル、引用グラフによってこの研究に関連する記事。

並び替え
Same author

PyTEA-O: a Python implementation of Two-Entropies Analysis for protein sequence variation analysis.

Bioinformatics (Oxford, England)·2026
Same author

Corrigendum to "Impact of cancer-associated mutations in CC chemokine receptor 2 on receptor function and antagonism" [Biochem. Pharmacol. 208 (2023) 115399].

Biochemical pharmacology·2023
Same author

Papyrus: a large-scale curated dataset aimed at bioactivity predictions.

Journal of cheminformatics·2023
Same author

Impact of cancer-associated mutations in CC chemokine receptor 2 on receptor function and antagonism.

Biochemical pharmacology·2022
Same author

Pan-cancer functional analysis of somatic mutations in G protein-coupled receptors.

Scientific reports·2022
Same author

GenUI: interactive and extensible open source software platform for de novo molecular generation and cheminformatics.

Journal of cheminformatics·2021

関連する実験動画

Updated: May 17, 2026

Mutagenesis and Functional Selection Protocols for Directed Evolution of Proteins in E. coli
09:01

Mutagenesis and Functional Selection Protocols for Directed Evolution of Proteins in E. coli

Published on: March 16, 2011

30.7K

PyVADesign:大きなミュータントライブラリの一段階生成のためのPythonベースのクローンツール

R C M Kuin1,2, M H Lamers2, G J P van Westen2

  • 1Computational Drug Discovery, Medicinal Chemistry, Leiden University, Leiden, 2333 CC, the Netherlands.

Bioinformatics (Oxford, England)
|September 1, 2025
PubMed
まとめ
この要約は機械生成です。

PyVADesignは新しいPythonパッケージで タンパク質変異体の作成を簡素化しています この費用対効果の高いツールは 効率的なタンパク質工学のために 二重鎖のDNA断片を設計し オーダーするのに 研究者を助けます

さらに関連する動画

Directed Evolution Method in Saccharomyces cerevisiae: Mutant Library Creation and Screening
10:50

Directed Evolution Method in Saccharomyces cerevisiae: Mutant Library Creation and Screening

Published on: April 1, 2016

11.0K
A Simple and Efficient Approach to Construct Mutant Vaccinia Virus Vectors
09:16

A Simple and Efficient Approach to Construct Mutant Vaccinia Virus Vectors

Published on: October 30, 2016

11.4K

関連する実験動画

Last Updated: May 17, 2026

Mutagenesis and Functional Selection Protocols for Directed Evolution of Proteins in E. coli
09:01

Mutagenesis and Functional Selection Protocols for Directed Evolution of Proteins in E. coli

Published on: March 16, 2011

30.7K
Directed Evolution Method in Saccharomyces cerevisiae: Mutant Library Creation and Screening
10:50

Directed Evolution Method in Saccharomyces cerevisiae: Mutant Library Creation and Screening

Published on: April 1, 2016

11.0K
A Simple and Efficient Approach to Construct Mutant Vaccinia Virus Vectors
09:16

A Simple and Efficient Approach to Construct Mutant Vaccinia Virus Vectors

Published on: October 30, 2016

11.4K

科学分野:

  • 分子生物学
  • タンパク質工学
  • バイオ情報学

背景:

  • タンパク質の機能を理解するには 多様なタンパク質の変異を生成することが重要です
  • ミュータントを生成する伝統的な方法は 時間がかかり 費用がかかります
  • 合成された二重鎖DNA (dsDNA) 断片の挿入は,突然変異のライブラリを作成するためのより速く,より安価な代替案を提供します.

研究 の 目的:

  • タンパク質の変異を目的とした dsDNA 断片を設計するためのアクセスしやすく効率的なツールを開発する.
  • タンパク質の変異を 簡素化する
  • タンパク質工学に伴うコストと時間を 削減するためです

主な方法:

  • dsDNA断片を設計するための Python パッケージである PyVADesign の開発.
  • 効率的なクローニングのための突然変異のクラスタリングの実施.
  • プラズミドの製造と配列の自動設計

主要な成果:

  • PyVADesignは,単純で費用対効果の高い dsDNA 断片の設計と注文を容易にする.
  • このパッケージは,クローニンググループを通して,標的のプラズミドに突然変異の効率的な交換を可能にします.
  • プラズミドの準備とシーケンシングの両方の自動化されたプライマー設計が統合されています.

結論:

  • PyVADesignは タンパク質工学や分子生物学の研究者にとって 価値あるリソースです
  • ソフトウェアは効率を高め タンパク質変異体ライブラリを生成するコストを削減します
  • PyVADesignはオープンソースであり,科学界でアクセシビリティとさらなる開発を促進します.