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Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

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Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
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Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

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Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
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The early pioneers of microscopy opened a window into the invisible world of microorganisms. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes that leveraged nonvisible light, such as fluorescence microscopy that uses an ultraviolet light source and electron microscopy that uses short-wavelength electron beams. These advances significantly improved magnification, image resolution, and contrast. By comparison, the...
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Two-dimensional (2D) microscopy encompasses a range of optical techniques that capture images within a single focal plane, offering detailed representations of microscopic structures. These techniques are essential in biological and medical research, enabling the visualization of cellular and subcellular structures with different levels of contrast and specificity.There are several major types of 2D microscopy, each with strengths and applications.Bright-Field MicroscopyBright-field microscopy...
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Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...
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Updated: May 6, 2026

Imaging and Quantifying Mitochondrial Morphology in C. elegans During Aging
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ダイナミックおよび三次元光顕微鏡によるミトコンドリア形態の変化の理解

Sholto de Wet1, Rensu Theart2, Ben Loos3

  • 1Department of Physiology, Stellenbosch University; Department of Biochemistry, University of Toronto; sholto.dewet@utoronto.ca.

Journal of visualized experiments : JoVE
|September 1, 2025
PubMed
まとめ

この研究では,ミトコンドリア イベント ローカリザー (MEL) ツールを使用してミトコンドリア ダイナミクスを分析する方法を紹介しています. この3Dイメージングアプローチは ストレスや治療にミトコンドリアネットワークがどのように反応するかを 理解するのに役立ちます

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Last Updated: May 6, 2026

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科学分野:

  • 細胞生物学
  • ミトコンドリア動力学
  • バイオイメージング

背景:

  • ミトコンドリアは 細胞の生存に不可欠な ダイナミックな臓器細胞です
  • ミトコンドリアネットワークは 分裂と融合によって絶えず再構成されます
  • ストレスや薬物に対する 細胞の反応を研究する上で 鍵となるのは このダイナミクスを理解することです

研究 の 目的:

  • ミトコンドリア イベント ロカライザー (MEL) の画像の準備のためのパイプラインを記述します.
  • ミトコンドリアネットワークのダイナミクスの3Dと時間解析を可能にします
  • ミトコンドリアの分裂と融合について 洞察を提供するためです

主な方法:

  • MELツールのイメージ準備パイプラインを開発しました.
  • ミトコンドリアネットワークを視覚化するために光画像を用いた.
  • ミトコンドリア動態の3D分析のためのMEL ImageJプラグインを適用しました.

主要な成果:

  • このパイプラインは,MELの画像作成を標準化します.
  • ミトコンドリアの分裂と融合の量的な分析を可能にします.
  • 時間の経過とともにミトコンドリアネットワークの再構築の理解を容易にする.

結論:

  • 記述されたパイプラインはミトコンドリア動態の分析を強化します.
  • ミトコンドリアネットワークの振る舞いを より深く知ることができます
  • ミトコンドリアのダイナミクスを理解することは 細胞の健康と疾患の研究に不可欠です