ミトコンドリアのAOX活性化が,スクレロチニア・スクレロチオラムにおけるパイリベンカーブ抵抗を誘発する:細胞交換と非標的部位メカニズム
Chenyan Zhou1, Qing Liu1, Jie Xie1
1College of Horticulture and Landscape, Tianjin Agricultural University, Tianjin 300392, China.
Journal of agricultural and food chemistry
|September 2, 2025
まとめ
菌媒体の変異により,スクレロチニア・スクレロチオラムでピリベンカーブに耐性のある変異体が発生した. 代替オキシダース (SsAox) 遺伝子の過剰発現により,この耐性が生じ,真菌の成長と呼吸に影響する.
科学分野:
- 植物病理学
- 菌類学
- 生物化学
背景:
- 農薬に対する耐性は 農業において懸念される問題となっています
- 耐性の分子メカニズムを理解することは 効果的な病気管理戦略の開発に不可欠です
- ピリベンカーブは,スクレロチニア・スクレロチオラムのような真菌によって引き起こされる植物病を制御するために使用される殺菌剤です.
研究 の 目的:
- スクレロチニア・スクレロチオラムのピリベンカーブ耐性に基づくメカニズムの調査
- 菌類殺菌剤耐性に関連した遺伝的および分子的要因を特定する.
- ピリベンカーブに耐性のある変異体の 生理学的および形態学的変化を分析する.
主な方法:
- 菌媒体の変異により 耐性のある変異体が生まれます
- 親種と変異種を比較した分析
- 生理学的測定 (胞の成長,硬化形成,呼吸)
- 分子分析 (遺伝子シーケンシング,RT-PCR,タンパク質発現)
- 分子ドッキングシミュレーション
主要な成果:
- ピリベンカーブに耐性のある変異体は,成長の低下,硬化体の形成の障害,ミトコンドリア機能障害を示した.
- 標的となるSscytbまたはSsaox遺伝子は見つかりませんでした.
- Ssaox遺伝子発現とタンパク質濃度の大幅なアップレギュレーション
- 分子ドッキングにより,ピリベンカーブがSsAoxドメインに結合することが示された.
- RH1-3変異体は 異なった形状の異常を示した
結論:
- 代替オキシダース (SsAox) 遺伝子の過剰発現は,スクレロチニア・スクレロチオラムにピリベンカーブ耐性を与える.
- SsAoxは真菌駆除剤耐性メカニズムにおいて重要な役割を果たします.
- この発見は,真菌駆除剤に対する耐性経路と病気の制御のための潜在的な標的についての洞察を提供します.
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