関連する実験動画
Updated: Sep 9, 2025

07:59
Spectrophotometric Methods for the Study of Eukaryotic Glycogen Metabolism
Published on: August 19, 2021
3.9K
ポリフォスファートキナーゼによるATP再生 効率的なオリゴサカリド合成 グリコシドリン酸化物によって触媒化
Xiaocong Wu1, Shuang Xing1, Di Ma1
1National Glycoengineering Research Center, Shandong Center of Technology Innovation for Carbohydrate, NMPA Key Laboratory for Quality Research and Evaluation of Carbohydrate-Based Medicine, Shandong University, Qingdao, Shandong 266237, China.
ACS chemical biology
|September 2, 2025
まとめ
この研究では,ポリフォスファートキナーゼ (PPK) を使用したオリゴサカリド合成のためのATPフリー酵素系を導入します. この費用対効果の高い方法は,単純な糖から多様な複雑な炭水化物をスケーラブルに生産することを可能にします.
科学分野:
- 生物化学
- 炭水化物の化学
- 酵素学
背景:
- グリコシドリン酸化酵素 (GPases) はオリゴサカリド合成に不可欠ですが,通常はATP依存キナーゼが必要です.
- 外因的なATPとキナーゼ媒介のリン酸化による依存は,酵素による炭水化物合成の実践的および経済的限界を提示する.
研究 の 目的:
- 効率的で費用対効果の高いオリゴサカリド合成のためのATPフリー酵素システムを開発する.
- このシステムの広範な適用性を様々なグリコシドリン酸化物とターゲットオリゴサッカライドで実証する.
主な方法:
- ポリフォスファートキナーゼ (PPK) とグリコシドフォスフォリラーゼ (GPases) と砂糖1-キナーゼを統合して,ATP再生システムを"in situ"にします.
- フォスファートドナーとしてポリフォスファートを利用し,外因的なATPの必要性を排除します.
- ラクト-N-バイオースI,ガラクト-N-バイオース,N-グリカン核トリサカリド,およびβ-マノシドを含む多様なオリゴサカリドのワンポット合成.
主要な成果:
- 40%から92%の収量で様々なオリゴサッカライドの合成を成功させた.
- PPK結合システムは,効率的なATP再生のために最小のAMP (<0. 05equiv) を要求した.
- ギャラクトシド,グルコサミニド,マノシドに作用するGPasesとの広範な互換性が実証されています.
結論:
- 開発されたATPフリー酵素戦略は,オリゴサッカリドの生産のためのスケーラブルで費用対効果の高いプラットフォームを提供します.
- このアプローチは,従来のATPに依存するメソッドの限界を回避し,コストを削減し,合成プロセスを簡素化します.
- このシステムの汎用性は,複雑な炭水化物の酵素合成をサポートし,グリコバイオロジーの研究と応用を進めています.
関連する概念動画
Biosynthesis of Polysaccharides
86
Polysaccharides such as glycogen and starch are synthesized from nucleoside diphosphate sugars, primarily uridine diphosphate glucose (UDPG) and adenosine diphosphate glucose (ADPG). These activated glucose donors act as key intermediates in carbohydrate metabolism and biosynthesis. UDPG primarily involves glycogen synthesis in animals and many bacteria, while ADPG plays a fundamental role in starch synthesis in plants and certain bacteria.UDPG is formed when glucose-1-phosphate reacts with...
86
ATP Energy Storage and Release
11.2K
ATP is a highly unstable molecule. Unless quickly used to perform work, ATP spontaneously dissociates into ADP and inorganic phosphate (Pi), and the free energy released during this process is lost as heat. The energy released by ATP hydrolysis is used to perform work inside the cell and depends on a strategy called energy coupling. Cells couple the exergonic reaction of ATP hydrolysis with endergonic reactions, allowing them to proceed.
One example of energy coupling using ATP involves a...
One example of energy coupling using ATP involves a...
11.2K
Glycolysis: Preparatory Phase
14.4K
In cellular metabolism (the complete breakdown of glucose to extract energy), glycolysis is the first step. Glycolysis takes place in the cytoplasm of both prokaryotic and eukaryotic cells. Glucose enters heterotrophic cells in two ways. One method is through secondary active transport, where the transport takes place against the glucose concentration gradient. The other mechanism uses a group of integral proteins called GLUT proteins, also known as glucose transporter proteins. These...
14.4K
ATP and Macromolecule Synthesis
6.1K
Biological macromolecules are organic compounds, predominantly composed of carbon atoms. The carbon atoms are covalently bonded with hydrogen, oxygen, nitrogen, and other minor elements. There are four major biological macromolecule classes: carbohydrates, lipids, proteins, and nucleic acids.
Most macromolecules are composed of single subunits, or building blocks, called monomers. The monomers combine with each other using covalent bonds to form larger molecules known as polymers.
Conversion of...
Most macromolecules are composed of single subunits, or building blocks, called monomers. The monomers combine with each other using covalent bonds to form larger molecules known as polymers.
Conversion of...
6.1K
Other Glycolytic Pathways
211
The pentose phosphate pathway (PPP) operates in parallel with glycolysis, facilitating the metabolism of both pentoses and glucose. This pathway consists of two distinct phases: the oxidative and non-oxidative phases. While it does not directly generate ATP, the intermediates formed during the process can integrate into glycolysis, contributing to cellular energy metabolism when required.Oxidative Phase: NADPH ProductionThe oxidative phase of the pentose phosphate pathway is primarily...
211
Energy-releasing Steps of Glycolysis
141.1K
Glycolysis is divided into two phases based on whether energy is utilized or released. While the first phase consumes ATP, the second phase produces energy in the form of ATP and NADH. The energy is released over a sequence of reactions that turns G3P into pyruvate. The energy-releasing phase—steps 6-10 of glycolysis—occurs twice, once for each of the two 3-carbon sugars produced during steps 1-5 of the first phase.
The first energy-releasing step—the 6th step of glycolysis...
The first energy-releasing step—the 6th step of glycolysis...
141.1K

