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Updated: May 5, 2026

Protocols for Implementing an Escherichia coli Based TX-TL Cell-Free Expression System for Synthetic Biology
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APEX:Escherichia coliにおける自動タンパク質発現

Martyna Kasprzyk1, Michael A Herrera1,2, Giovanni Stracquadanio1

  • 1School of Biological Sciences, The University of Edinburgh, Edinburgh EH9 3BF, U.K.

ACS synthetic biology
|September 2, 2025
PubMed
まとめ

自動タンパク質発現 (APEX) は,オペントロン OT-2 プラットフォームを使用して,エシェリキア大腸 (E. coli) の再結合タンパク質の生産を効率化します. この自動化されたシステムは,微生物のタンパク質発現の高通量スクリーニングの効率と再現性を高めます.

キーワード:
オートメーション高通量スクリーニング液体処理ロボットタンパク質発現プロトコル標準化について

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