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関連する概念動画

Pre-mRNA Processing: RNA Splicing01:36

Pre-mRNA Processing: RNA Splicing

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5.4K
RNA Splicing01:32

RNA Splicing

56.9K
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
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Chromatin Structure Regulates pre-mRNA Processing02:41

Chromatin Structure Regulates pre-mRNA Processing

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In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...
7.2K
Alternative RNA Splicing02:18

Alternative RNA Splicing

21.7K
Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
21.7K
Pre-mRNA Processing: Modification of pre-mRNA Ends01:35

Pre-mRNA Processing: Modification of pre-mRNA Ends

9.8K
In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a cap to the 5' end of the growing transcript. In this process, a 5' phosphate is replaced by modified guanosine that has a methyl group attached (7-methyl guanosine). This 5' cap helps...
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Chromatin Structure and RNA Splicing02:41

Chromatin Structure and RNA Splicing

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Updated: Sep 9, 2025

Using the E1A Minigene Tool to Study mRNA Splicing Changes
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スプライシングと3'処理の競争がヒトのトランスクリプトームを形作る

Lindsey V Soles, Shuangyu Li, Liang Liu

    bioRxiv : the preprint server for biology
    |September 2, 2025
    PubMed
    まとめ
    この要約は機械生成です。

    mRNA前スプライシングを妨害すると,何千もの内部ポリアデニレーション部位が活性化し,早めに転写が終了します. 逆に,3'処理を抑制すると,スプライシングが全般的に強化され,トランスクリプトームを形作る競争的な相互作用が明らかになる.

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    A Reporter Based Cellular Assay for Monitoring Splicing Efficiency
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    Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells
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    関連する実験動画

    Last Updated: Sep 9, 2025

    Using the E1A Minigene Tool to Study mRNA Splicing Changes
    10:25

    Using the E1A Minigene Tool to Study mRNA Splicing Changes

    Published on: April 22, 2021

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    A Reporter Based Cellular Assay for Monitoring Splicing Efficiency
    08:53

    A Reporter Based Cellular Assay for Monitoring Splicing Efficiency

    Published on: September 15, 2021

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    Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells
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    Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells

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    科学分野:

    • 分子生物学
    • 遺伝子規制
    • トランスクリプション・コントロール

    背景:

    • ユカリオット前mRNA処理は,協調されたスプライシングと3'処理を含む.
    • この調整を制御する正確なメカニズム,特に3'処理 (テレスクリプト) を阻害するU1 snRNPの役割は完全に理解されていません.

    研究 の 目的:

    • スプライシングと3'処理の相互作用を調査する.
    • 3'処理と転写終結に影響を与えるスプライシング因子のメカニズムを解明する.

    主な方法:

    • スプライシングを妨げるために,主要なスプライシング因子 (U1 snRNP,U2 snRNP,U2AF,SF3b) をターゲットにする.
    • 内部ポリアデニレーション (IPA) 部位の活性化を分析する.
    • 遺伝子体内の早すぎる転写終了を評価する.
    • 3'処理を阻害し,スプライシングへの影響を観察する.

    主要な成果:

    • 特にU1 snRNPのスプライシング障害は,数千のIPAサイトを活性化しました.
    • スプライシング阻害は,IPA結合および独立経路による広範な早期転写終結につながった.
    • IPAサイトには異なるスプレッシングファクタが適用されます.
    • 3'処理の阻害により,グローバル・エンハンス・スプライシングが行われる.

    結論:

    • スプライシングと3'処理は競合するプロセスです.
    • これらのプロセスは 遺伝子発現を制御するために 転写と交差する.
    • 発見はテレスクリプトモデルに挑戦し,より広範な規制ネットワークを提案しています.