転写因子-sRNA媒介の二重負のフィードバックループは,病原体特異的なクオラムセンシング遺伝子の制御を与える

Ameya A Mashruwala^1,2,3, Kaitlin Decker^1,2,4, Chenyi Fei^1,2,5

¹Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544, USA.

bioRxiv : the preprint server for biology

|September 2, 2025

まとめ

細菌はクオラムセンシングを使ってコミュニケーションをとります Vibrio choleraeでは,LuxTはVqmRを抑制し,VqmRはLuxTを抑制し,バイオフィルム形成と宿主コロニー化に不可欠なフィードバックループを形成する.

科学分野:

微生物学
バクテリアの伝達
遺伝子調節

背景:

クオラムセンシングは細菌が信号分子を介して行動を調整することを可能にします.
Vibrio choleraeでは,VqmAがVqmRの小さな調節性RNAの生成を活性化し,バイオフィルム形成に影響を与える.

研究の目的:

Vibrio cholerae の DPO-VqmA-VqmR 信号伝達経路を抑制する物質を特定する.
この経路におけるLuxT転写因子の規制的役割を調査する.

主な方法:

vqmRの転写におけるLuxTの役割の特徴
luxTのVqmRの転写後の調節の分析
相互制御に欠かせないLuxTのN端の領域の識別
LuxT制御遺伝子のセットを定義する.

主要な成果:

LuxTはvqmRの転写を抑制し,VqmRは転写後の luxTの翻訳を抑制し,二重負のフィードバックループを生成する.
相互制御は,LuxTのN端8アミノ酸によって媒介され,DNA結合にも影響する.
この調節回路は,Vibrio choleraeおよび関連種に特異的です.
LuxTはビブリオ・コレラのバイオフィルム形成を促進する.

結論:

LuxTとVqmRの間の新しいフィードバックループは,Vibrio choleraeにおけるクオラムセンシングを調節する.
この回路は,LuxTのN端領域に依存し,バイオフィルム形成と宿主コロニー化に不可欠です.
特定された規制メカニズムは,ビブリオ属内で進化的に制限されています.

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