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関連する概念動画

Master Transcription Regulators02:23

Master Transcription Regulators

Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a  complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
Transcription Attenuation in Prokaryotes02:42

Transcription Attenuation in Prokaryotes

Transcriptional attenuation occurs when RNA transcription is prematurely terminated due to the formation of a terminator mRNA hairpin structure.  Bacteria use these hairpins to regulate the transcription process and control the synthesis of several amino acids including histidine, lysine, threonine, and phenylalanine. Transcription attenuation takes place in the non-coding regions of mRNA.
There are several different mechanisms used to attenuate transcription. In ribosome mediated...
RNA-seq03:21

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...
Cell Specific Gene Expression01:58

Cell Specific Gene Expression

Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
Master Transcription Regulators02:23

Master Transcription Regulators

Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a  complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
mRNA Stability and Gene Expression02:51

mRNA Stability and Gene Expression

The structure and stability of mRNA molecules regulates gene expression, as mRNAs are a key step in the pathway from gene to protein. In eukaryotes, the half-life of mRNA varies from a few minutes up to several days. mRNA stability is essential in growth and development. The absence of the proteins regulating its stability, such as tristetraprolin in mice, can cause systemic issues, including bone marrow overgrowth, inflammation, and autoimmunity.
Cis-acting Elements involved in mRNA stability

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Updated: May 8, 2026

Transcriptome Analysis of Single Cells
07:27

Transcriptome Analysis of Single Cells

Published on: April 25, 2011

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単細胞のトランスクリプトームに欠けているデータは,トランスクリプションのシフトを明らかにする.

Ruizhe Chen, Yu-Che Chung, Beth Kelly

    bioRxiv : the preprint server for biology
    |September 2, 2025
    PubMed
    まとめ

    新しいスコアメソッドは,欠落したデータを分析し,細胞の優先順位付けを支援し,Dictyostelium discoideumの発達における代謝のシフトを明らかにすることによって,異なる単細胞のトランスクリプトームを特定します.

    科学分野:

    • 単細胞ゲノム
    • 計算生物学
    • 発達生物学

    背景:

    • 単細胞RNAシーケンシング (scRNA-seq) は,大規模なトランスクリプトームプロファイリングを可能にします.
    • ダウンストリーム分析のためのセル優先順位は,技術的な変動と複雑なデータ構造のために困難です.
    • 既存の方法は,クラスタリング,標準化,および次元縮小技術によって混同されることが多い.

    研究 の 目的:

    • 偏りのない細胞優先順位付けのための新しいスコア付けアプローチを開発する.
    • 通常のscRNA-seq処理ステップとは独立して,欠落したデータパターンを基に異なるトランスクリプトームを識別する.
    • 後の分析のために強固な特徴選択を可能にします.

    主な方法:

    • シングル・セル・トランスクリプトームの欠落したデータパターンを利用したスコアリングアプローチが開発された.
    • この方法は,クラスタリング,正規化,および次元縮小戦略から独立するように設計されました.
    • このアプローチは,Dictyostelium discoideumからのトランスクリプトミックのデータを分析するために適用されました.

    主要な成果:

    • スコアリング方法は,データセット内の異なるトランスクリプトームを成功裏に特定しました.

    さらに関連する動画

    Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues
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    Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues

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    An Approach to Study Shape-Dependent Transcriptomics at a Single Cell Level
    06:02

    An Approach to Study Shape-Dependent Transcriptomics at a Single Cell Level

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    関連する実験動画

    Last Updated: May 8, 2026

    Transcriptome Analysis of Single Cells
    07:27

    Transcriptome Analysis of Single Cells

    Published on: April 25, 2011

    30.1K
    Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues
    10:12

    Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues

    Published on: January 10, 2019

    18.7K
    An Approach to Study Shape-Dependent Transcriptomics at a Single Cell Level
    06:02

    An Approach to Study Shape-Dependent Transcriptomics at a Single Cell Level

    Published on: November 2, 2020

    5.8K

  • 偏りのない細胞優先順位と特徴の選択が達成されました.
  • Dictyostelium discoideumの分析は,発達状態の移行に関連した重要な代謝変化を明らかにした.

    • 結論:

    • 開発されたスコアリングアプローチは,単細胞トランスクリプトミクスの細胞優先順位付けのための堅牢で偏りのない方法を提供します.
    • この技術は,データ処理のステップによって混乱する伝統的な方法の限界を克服します.
    • Dictyostelium discoideumの発見は,発達代謝の変化などの生物学的洞察を明らかにするためのメソッドの有用性を強調しています.