カサバのパルプと窒素サプリメントを用いたBacillus amyloliquefaciensKW8による抗酸化エクソポリサハリドの産生に関するデータ
Nantaporn Sutthi1,2, Thipphiya Karirat3, Piyathida Promjamorn3
1Department of Agricultural Technology, Faculty of Technology, Mahasarakham University, Maha Sarakham, 44150, Thailand.
Data in brief
|September 2, 2025
まとめ
この研究では,酵母抽出物とカサワのパルプを用いてバチルス・アミロリケファシエンスによる抗酸化物質の産生を最適化しました. この研究は,農業廃棄物から持続可能なバイオ製品の開発を強調しています.
科学分野:
- バイオテクノロジー
- 微生物学
- 生物化学
背景:
- カサバのパルプのような農業廃棄物は 微生物発酵のための持続可能な炭素源です
- エキソポリサッカリド (EPS) は,食品および医薬品における潜在的な用途を持つ重要な抗酸化特性を示しています.
- バシルス・アミロリケファシエンス (Bacillus amyloliquefaciens) は,EPSを含む生物活性化合物の生産者として知られています.
研究 の 目的:
- Bacillus amyloliquefaciens KW8による抗酸化エキゾポリサカリド (EPS) 生産のための最適な窒素源を決定する.
- 発酵のパラメータを最適化して,EPSの収穫量と抗酸化活性を増強する.
- カサバのパルプの農業廃棄物を 有価なバイオ製品に変換する方法を評価する.
主な方法:
- 酵母抽出物が最適であるかどうかを判別する.
- 中央複合設計 (CCD) の応答表面方法論 (RSM) を適用して,pH,インオキュラムサイズ,炭素と窒素 (C/N) の比率,および振動速度を最適化します.
- スキャニング電子顕微鏡 (SEM) によるEPSの収穫量,媒体のpH,減糖,細菌群,および構造特性の分析.
主要な成果:
- イースト抽出物は,EPSの生産に最も効果的な窒素源であると特定されました.
- 最適な条件により,EPSの産生と抗酸化作用が強化された.
- 生成されたEPSの生物活性化合物含量と抗酸化能力 (フェノール,フラボノイド,FRAP,ヒドロキシル基,DPPH除去活性) を特徴づけました.
結論:
- バチルス・アミロリケファシエンス (Bacillus amyloliquefaciens) は カサワのパルプを 効率的に 抗酸化物質のEPSに変換します
- 発酵パラメータの最適化により,EPSの収穫量と生物活性が著しく改善されます.
- このバイオプロセスは 農業廃棄物を高価値の製品に 価値づけることで 持続可能な開発目標を支援します
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