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Updated: Jun 17, 2026

Identification of Protein Interaction Partners in Mammalian Cells Using SILAC-immunoprecipitation Quantitative Proteomics
12:53

Identification of Protein Interaction Partners in Mammalian Cells Using SILAC-immunoprecipitation Quantitative Proteomics

Published on: July 6, 2014

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ヘラ細胞の洗剤耐性膜内のフロチリン-2相互作用体の定量タンパク質データ

Anita Hryniewicz-Jankowska1, Michał Tracz2, Ilona Opiełka1

  • 1Department of Cytobiochemistry, Faculty of Biotechnology, University of Wrocław, ul. Joliot-Curie 14a, 50-383, Wrocław, Poland.

Data in brief
|September 2, 2025
PubMed
まとめ

膜ラフト内のフロチリン2タンパク質の相互作用は,BioID近接ラベリングを用いてマッピングされた. この研究は,癌に関連するシグナル伝達経路を理解するために重要な信号伝達と膜生殖における役割を明らかにする新しいフロチリン-2相互作用体を特定しました.

キーワード:
バイオIDの近接相互作用DRM についてフロチリン-2LC-MS/MS についてラベルなしの定量プロテオミック分析

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Quantification of Protein Interaction Network Dynamics using Multiplexed Co-Immunoprecipitation

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Label-Free Immunoprecipitation Mass Spectrometry Workflow for Large-scale Nuclear Interactome Profiling

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Detergent Purification of Membrane Proteins01:18

Detergent Purification of Membrane Proteins

Detergents are used to purify the integral proteins of the membrane. The hydrophobic portion of the detergent can replace membrane phospholipids while solubilizing the membrane proteins. When detergent monomers reach a specific concentration in a solution called critical micelle concentration (CMC), they form micelles. Above CMC, the concentration of the detergent monomers remains in equilibrium with the micelle. The number of detergent monomers present in the CMC varies for each detergent, and...