PBP1a の単一のアミノ酸は,Streptococcus suis の高いレベルのペニシリンとアモキシシリン抵抗性を誘発する

Zeren Peng¹, Jianping Wang², Shun Kang¹

¹MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210014, China;; Key Lab of Animal Bacteriology, Ministry of Agriculture and Rural Affairs, Nanjing 210014, China;; WOAH Reference Lab for Swine Streptococcosis, Nanjing 210014, China.

International journal of antimicrobial agents

|September 2, 2025

まとめ

ペニシリンとアモキシシリンの耐性が上昇しています PBP1aの特定の変異 (S695A) は抗生物質の結合を著しく減少させ,この重要な動物性病原体における高レベルの耐性を説明する.

科学分野:

微生物学
分子生物学
抗菌剤に対する耐性

背景:

Streptococcus suisは,人間と豚に影響を与える重要な動物性病原体です.
ベータ・ラクタム抗生物質が主な治療法ですが耐性菌が増えています
S. suis のベータ・ラクタム抵抗性のメカニズムは十分に理解されていません.

研究の目的:

ペニシリンとアモキシシリンに抵抗する分子メカニズムの調査
抗生物質耐性につながる特定の遺伝子変異を特定する.

主な方法:

中国産のS. suis菌株534種を分析した (2005年から2024年).
ペニシリン結合タンパク質 (PBP) のアミノ酸配列の調整
分子ドッキング,アサイレーション効率測定,サイト指向型変異,MICテスト.

主要な成果:

PBP1aの変異 (S695A) は,高いレベルのペニシリンおよびアモキシシリン耐性における重要な要因として特定されました.
この変異は,これらの抗生物質に対するPBPの結合親和性を著しく低下させる.
耐性菌株の変異を逆転させると耐性レベルが劇的に低下します

結論:

PBP1aのS695A変異は,S. suisにおけるペニシリンおよびアモキシシリン耐性の主要な要因である.
このメカニズムの理解は,この動物性病原体における抗菌剤耐性の増大と闘うために極めて重要です.

キーワード:

ストレプトコッカス・スイスアモキシチリンペニシリンペニシリン結合タンパク質

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