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Updated: Sep 9, 2025

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遺伝学的に異なるサケ酵母の優れた発酵性能を支えるメカニズムにおけるロカス異質性

Kotaro Mori¹, Taisuke Seike², Takeshi Akao³

¹General Research Laboratory, Kiku-Masamune Sake Brewing Co. Ltd., 1-8-6 Uozaki-nishimachi, Higashinada-ku, Kobe, Hyogo 658-0026, Japan; Department of Bioinformatic Engineering, Graduate School of Information Science and Technology, Osaka University, 1-5 Yamadaoka, Suita, Osaka 565-0871, Japan.

Journal of bioscience and bioengineering

|September 2, 2025

まとめ

古典的なサケ酵母Km67は,Rim15pのシグナル伝達が低下したため,現代の株とは異なり,発酵が強化されています. この研究は酵母発酵メカニズムにおける場所の異質性を明らかにしている.

科学分野:

微生物学
酵母遺伝学
発酵科学

背景:

サケ酵母 (Saccharomyces cerevisiae) は優れた発酵能力を持っています
現代のサケ酵母K701の能力は知られているが,古典的な株はあまり研究されていない.
古典的なサケ酵母Km67の優れた発酵メカニズムは調査が必要です.

研究の目的:

古典的なサケ酵母Km67における優れた発酵の遺伝的および生理学的基礎を解明する.
Km67とX2180とK701の検査株を比較する
サケ酵母における発酵の調節メカニズムを特定する.

主な方法:

遺伝子と生理学的特性の比較分析
酵母トランスクリプトームのRNA配列解析
Rim15pとタンパク質フォスファタゼ2A (PP2A^B55δ) を含むシグナル伝達経路の遺伝子検査

主要な成果:

Km67は,より高い発酵率と相関する,サケマッシュの静止状態に入らなかった.
Rim15p信号による発酵抑制の障害がKm67で観察された.
CDC55の削除 (Rim15pのダウンストリーム) は発酵を減少させましたが,他の株よりも少なく,他の要因を示唆しています.

結論:

Km67の優れた発酵は,Rim15p媒介の抑制とPP2A^B55δの活性の変化と関連しています.
未確認の要因は,PP2A^B55δまたはKm67のRim15pとは独立して発酵を調節する可能性がある.
Km67 と K701 のような遺伝学的に遠く離れたサケ酵母間の発酵メカニズムには有意な場所の異質性がある.

キーワード:

細胞壁の調節 RIM15 について RNAシーケンシングサケの発酵サケ酵母ストレス反応

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