タイレニアの木 (Hyla sarda) の染色体レベルでのゲノムアセンブリ
Josephine R Paris1,2, Roberta Bisconti3, Andrea Chiocchio4
1Department of Health, Life and Environmental Sciences, University of L'Aquila, Coppito, Italy.
Scientific data
|September 2, 2025
まとめ
ティレニアの木 (Hyla sarda) の高品質のゲノムアセンブリが作成されました. このリソースは,進化の範囲の拡大中に発生する現象的変化の遺伝的基礎を理解するのに役立ちます.
科学分野:
- ゲノミクス
- 進化生物学
- 両生類の研究
背景:
- ティレニアの木のカエル (Hyla sarda) は,範囲の拡大に関連した現象的変化を示しています.
- この変異の遺伝的基盤の理解は,参照ゲノムの欠如によって制限されています.
研究 の 目的:
- ハイラ・サルダの染色体レベルの参照ゲノムを組み立てるため
- 進化と保全の研究のためのゲノム資源を提供すること.
主な方法:
- ゲノムアセンブリのためにPacBio HiFi ロングリーズ,バイオナノ光学マップ,Hi-Cデータを活用した.
- ゲノムアセンブリと遺伝子アノテーションのための標準的なバイオ情報パイプラインを使用しています.
主要な成果:
- 13個の染色体を持つ4. 15Gbの染色体レベルのゲノムアセンブリが生成されました.
- 高い完全性スコア (BUSCO 94. 60%, k- mer 98. 30%) と,22847の注釈されたタンパク質コード遺伝子が達成されました.
- 複製要素は組み立てられたゲノムの約75%を占める.
結論:
- ハイラ・サルダの高品質のゲノムアセンブリは 進化と保全ゲノミクスの重要なリソースです
- 氷河による範囲の移転の際の遺伝子型-フェノタイプの相関に関する研究を容易にする.
- ハイラ・サルダの保存のための将来的な集団ゲノム研究を可能にします.
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