の腹部脂肪と卵産のフェノタイプの特徴の形成における転置可能な要素の役割の調査
Silu Hu1,2, Yi Luo3, Ying Chen3
1Livestock and Poultry Multi‑Omics Key Laboratory of Ministry of Agriculture and Rural Affairs, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, 611130, China.
BMC genomics
|September 2, 2025
まとめ
トランスポーザブルエレメント (TE) は,脂肪の蓄積や卵の産生などのウミガメの特徴に大きく影響し,遺伝的繁殖の新たな道を開きます. この研究では,多数のTE欠席ポリモルフィズム (TAP) を特定し,特定のTEを主要な現象型変異と関連付けました.
科学分野:
- ゲノミクスと動物の繁殖
- 分子生物学
- 定量遺伝学
背景:
- トランスポーザブル要素 (TE) は,ゲノム多様性の原動力として知られていますが,アヒルの表型特性におけるその役割は十分に研究されていません.
- 以前の研究は主に単一の核酸多形態 (SNP) に焦点を当て,TEsの潜在的な影響を無視した.
- TEの影響を理解することは,ゲスの遺伝的繁殖戦略を進めるために不可欠です.
研究 の 目的:
- シチュワンの白うさぎの TE欠乏ポリモルフィズム (TAP) を特定し,特徴づけること.
- TEと様々な現象特性の関連性,特に腹部脂肪の重さを TE全ゲノム関連性研究 (TE-GWAS) を使用して調査する.
- 同発現分析を通じて,TEsと卵産生に関連する遺伝子の相関性を探求する.
主な方法:
- 566匹のシチュアン白うさぎの全ゲノム再配列化により,157,044匹のTE欠乏ポリモルフィズム (TAP) が確認された.
- 腹部脂肪重量に焦点を当てた48の特徴について TE全ゲノム関連研究 (TE-GWAS) を実施した.
- 卵巣トランスクリプトームのデータで4つのの集団の共表現分析を行った.
主要な成果:
- CDCC171遺伝子内の TEと腹部脂肪の蓄積との間に,近くのSNPとの低結合不均衡 (LD) が有意な関連性が見つかりました.
- TEsは,特に長い末端リピート (LTR) と末端逆転リピート (TIR) の要素は,卵の生産に関与する遺伝子と有意な相関関係を示した.
- TEsは,SNPの伝統的なマーカーとは独立して,ガチョウの表型変異に大きく寄与していることが判明しました.
結論:
- 移植可能な要素はガチョウの表型変化の重要な原動力であり,脂肪の蓄積や卵の産生などの特徴に影響を与えます.
- TE欠席ポリモルフィズム (TAP) は,ハゲの多様性と特性を理解するための貴重な遺伝資源です.
- これらの発見は,の経済的に重要な特性を改善することを目的とした標的型繁殖プログラムのための新しい可能性を開きます.
関連する概念動画
Overview of Transposition and Recombination
16.1K
Transposons make up a significant part of genomes of various organisms. Therefore, it is believed that transposition played a major evolutionary role in speciation by changing genome sizes and modifying gene expression patterns. For example, in bacteria, transposition can lead to conferring antibiotic resistance. Movement of transposable elements within the genetic pool of pathogenic bacteria can aid in transfer of antibiotic-resistant genetic elements. In eukaryotes, transposons can carry out...
16.1K
Background and Environment Affect Phenotype
6.7K
Although the genetic makeup of an organism plays a major role in determining the phenotype, there are also several environmental factors, such as temperature, oxygen availability, presence of mutagens, that can alter an organism’s phenotype.
An example of how genetic background affects phenotype can be seen in horses. The Extension gene in horses is responsible for their coat color. A wild-type gene (EE) produces black pigment in the coat, while a mutant gene (ee) produces red pigment. A...
An example of how genetic background affects phenotype can be seen in horses. The Extension gene in horses is responsible for their coat color. A wild-type gene (EE) produces black pigment in the coat, while a mutant gene (ee) produces red pigment. A...
6.7K
Transposons
146
Transposons, or "jumping genes," are small mobile genetic elements (MGEs) that range from 700 to 40,000 base pairs in length. They are found in all organisms and can move within the same chromosome or transfer to different chromosomes. In some cases, transposons can also jump between different host DNA molecules, such as plasmids or viruses, contributing to genetic variability.Barbara McClintock first discovered these mobile genetic elements in the 1940s while studying maize genetics, and she...
146
Position-effect Variegation
6.5K
In 1928, a German botanist Emil Heitz observed the moss nuclei with a DNA binding dye. He observed that while some chromatin regions decondense and spread out in the interphase nucleus, others do not. He termed them euchromatin and heterochromatin, respectively. He proposed that the heterochromatin regions reflect a functionally inactive state of the genome. It was later confirmed that heterochromatin is transcriptionally repressed, and euchromatin is transcriptionally active chromatin.
6.5K
Transgenic Organisms
31.6K
Overview
31.6K
DNA-only Transposons
14.8K
DNA-only transposons are called autonomous transposons since they code for the enzyme transposase that is required for the transposition mechanism. Insertion of transposons can alter gene functions in multiple ways. They can mutate the gene, alter gene expression by introducing a novel promoter or insulator sequence, introduce new splice sites, and change the mRNA transcripts produced, or remodel chromatin structure.
The donor site from where the transposon is excised is either degraded or...
The donor site from where the transposon is excised is either degraded or...
14.8K


