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Updated: May 10, 2026

Scaffold-supported Transplantation of Islets in the Epididymal Fat Pad of Diabetic Mice
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Scaffold-supported Transplantation of Islets in the Epididymal Fat Pad of Diabetic Mice

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バイオミテック3Dスキャフォールドのインスリン産生INS-1細胞培養

Mikhail Parker1, Nataraja Sekhar Yadavalli2, Kristina Peranidze1,3

  • 1Nanostructured Materials Laboratory, University of Georgia, Athens, GA, 30602, USA. sminko@uga.edu.

Journal of materials chemistry. B
|September 3, 2025
PubMed
まとめ

研究者らは,インスリン産生INS-1細胞を培養するための新しい3Dポリキャプロラクトン・スキャフォルトを開発した. これらの骨組みは細胞の成長と インスリン生産をサポートし 組織工学の応用に最適な細胞シートを 作り出します

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関連する概念動画

Production of Pharmaceuticals01:30

Production of Pharmaceuticals

Industrial insulin production uses genetically engineered E. coli expressing a proinsulin gene controlled by a tryptophan promoter and containing a methionine linker for later cleavage. The cells also carry ampicillin resistance for selective growth. Seed cultures are stored at −80 °C and production begins by thawing a small amount to inoculate starter cultures, which are progressively scaled to a 50,000-L bioreactor. In the bioreactor, E. coli grow in nutrient-rich media under sterile, tightly...