Jove
Visualize
お問い合わせ
JoVE
x logofacebook logolinkedin logoyoutube logo
JoVEについて
概要リーダーシップブログJoVEヘルプセンター
著者向け
出版プロセス編集委員会範囲と方針査読よくある質問投稿
図書館員向け
推薦の声購読アクセスリソース図書館諮問委員会よくある質問
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experimentsアーカイブ
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教員リソースセンター教員サイト
利用規約
プライバシーポリシー
ポリシー

関連する概念動画

Nucleic Acid Structure01:25

Nucleic Acid Structure

6.9K
The pentose sugar in DNA is deoxyribose, while in RNA the pentose sugar is ribose. The difference between the sugars is the presence of the hydroxyl group on the ribose's second carbon and a hydrogen on the deoxyribose's second carbon. The phosphate residue attaches to the hydroxyl group of the 5′ carbon of one sugar and the hydroxyl group of the 3′ carbon of the sugar of the next nucleotide, which forms  a 5′ to 3′ phosphodiester linkage.
DNA Structure
DNA...
6.9K
The Replisome03:01

The Replisome

34.7K
DNA replication is carried out by a large complex of proteins that act in a coordinated matter to achieve high-fidelity DNA replication. Together this complex is known as the DNA replication machinery or the replisome.
The synthesis of the leading and lagging strands is a highly coordinated process. To explain this, the “Trombone model” was proposed by Bruce Alberts in 1980. The DNA loop formation starts when a primer is synthesized on the parent lagging strand. The loop grows with...
34.7K
Lagging Strand Synthesis01:59

Lagging Strand Synthesis

54.1K
During replication, the complementary strands in double-stranded DNA are synthesized at different rates. Replication first begins on the leading strand. Replication starts later, occurs more slowly, and proceeds discontinuously on the lagging strand.
There are several major differences between synthesis of the leading strand and synthesis of the lagging strand. 1) Leading strand synthesis happens in the direction of replication fork opening, whereas lagging strand synthesis happens in the...
54.1K
DNA Replication02:40

DNA Replication

50.8K
DNA replication involves the separation of the two strands of the double helix, with each strand serving as a template from which the new complementary strand is copied.  After replication, each double-stranded DNA includes one parental or “old” strand and one “new” strand. This is known as semiconservative replication. The resulting DNA molecules have the same sequence and are divided equally into the two daughter cells.
Replication in Prokaryotes
DNA replication...
50.8K
The DNA Replication Fork01:02

The DNA Replication Fork

36.7K
An organism’s genome needs to be duplicated in an efficient and error-free manner for its growth and survival. The replication fork is a Y-shaped active region where two strands of DNA are separated and replicated continuously. The coupling of DNA unzipping and complementary strand synthesis is a characteristic feature of a replication fork.   Organisms with small circular DNA, such as E. coli, often have a single origin of replication; therefore, they have only two replication...
36.7K
Replication in Prokaryotes02:35

Replication in Prokaryotes

88.8K
Overview
88.8K

こちらも読む

関連記事

共著者、ジャーナル、引用グラフによってこの研究に関連する記事。

並び替え
Same author

Spatially resolved profiling of extracellular vesicles in tissues with Spatial-EV-seq.

Nature biotechnology·2026
Same author

Lysosome-Targeting Chimeras (LYTACs): From Modular Design Principles to Diverse Therapeutic Applications.

Chemistry (Weinheim an der Bergstrasse, Germany)·2026
Same author

SpatioCell: deep integration of histology and spatial transcriptomics for profiling the cellular microenvironment at single-cell level.

Science bulletin·2026
Same author

Cyclic transformation of stable/metastable nucleic acid structures enables dynamic monitoring of ATP in living cells.

Chemical science·2026
Same author

Dual-Spatially Confined Assembly of DNA Nanowall Stiffens Tumor Cells to Enhance Adoptive T-Cell Immunotherapy.

Journal of the American Chemical Society·2026
Same author

Tracing Tumor-Derived Extracellular Vesicle Matrix Metalloproteinase 14 Using Dual-Target Orthogonal Barcoding-Based Microscale Thermophoretic Assays.

ACS nano·2026
Same journal

Deep Learning Network-Tailored Microenvironment Matching of 4D Bioprinting Bioactive Scaffolds for Bone Regeneration.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same journal

Autonomous High-Throughput Characterization of Liquid-Liquid Phase Behavior.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same journal

Laser Preset of MnO<sub>x</sub> Layer on High-Entropy Alloy Surface for Ampere-Level Ultra-Stable OER Performance.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same journal

PDGFRα<sup>+</sup>/Integrin α2<sup>+</sup> Fibroblasts Orchestrate Tumor Budding in Oral Squamous Cell Carcinoma via Mechano-Metabolic Symbiosis: E-Cadherin/Integrin α2β1 Adhesion and Mitochondrial Transfer.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same journal

Synergistic Ni Single Atoms/Nanoparticles on CeO<sub>2</sub> for High-Performance and Durable SOFC Hydrogen Electrodes.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same journal

A Review of Failure Modes and Safety Strategies of Lithium-Ion Batteries from Materials to Systems.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
関連記事をすべて見る

関連する実験動画

Updated: Sep 9, 2025

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation
09:26

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation

Published on: December 29, 2021

4.3K

高度配列のDNAフレームワークインターフェースは,効率的な酵素オリゴヌクレオチド合成を可能にします.

Kunjie Li1, Dongbao Tang2, Xiaoyun Lu3

  • 1The MOE Key Laboratory of Spectrochemical Analysis and Instrumentation, State Key Laboratory of Physical Chemistry of Solid Surfaces, Department of Chemical Biology, College of Chemistry and Chemical Engineering, Innovation Laboratory for Sciences and Technologies of Energy Materials of Fujian Province (IKKEM), Xiamen University, Xiamen, 361005, China.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)
|September 3, 2025
PubMed
まとめ
この要約は機械生成です。

研究者は酵素性オリゴヌクレオチド合成 (EOS) を改善するための3DDNAフレームワークを開発した. このナノスケールのインターフェースは 酵素のアクセシビリティとDNA合成の効率を高め DNA情報を正確に保存できます

キーワード:
DNA 情報の保存DNA合成効率合成生物学四面体DNAナノ構造

さらに関連する動画

Simple, Affordable, and Modular Patterning of Cells using DNA
08:59

Simple, Affordable, and Modular Patterning of Cells using DNA

Published on: February 24, 2021

4.2K
Protocol for the Solid-phase Synthesis of Oligomers of RNA Containing a 2'-O-thiophenylmethyl Modification and Characterization via Circular Dichroism
11:37

Protocol for the Solid-phase Synthesis of Oligomers of RNA Containing a 2'-O-thiophenylmethyl Modification and Characterization via Circular Dichroism

Published on: July 28, 2017

19.2K

関連する実験動画

Last Updated: Sep 9, 2025

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation
09:26

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation

Published on: December 29, 2021

4.3K
Simple, Affordable, and Modular Patterning of Cells using DNA
08:59

Simple, Affordable, and Modular Patterning of Cells using DNA

Published on: February 24, 2021

4.2K
Protocol for the Solid-phase Synthesis of Oligomers of RNA Containing a 2'-O-thiophenylmethyl Modification and Characterization via Circular Dichroism
11:37

Protocol for the Solid-phase Synthesis of Oligomers of RNA Containing a 2'-O-thiophenylmethyl Modification and Characterization via Circular Dichroism

Published on: July 28, 2017

19.2K

科学分野:

  • バイオテクノロジー
  • 分子生物学
  • ナノテクノロジー

背景:

  • DNA合成は生命科学において不可欠です
  • 酵素性オリゴヌクレオチド合成 (EOS) は,化学的方法よりも費用対効果と環境への配慮などの利点を提供しています.
  • 現在のEOS方法は,プライマーのアクセシビリティと酵素の阻害が限られているため,課題に直面しています.

研究 の 目的:

  • 効率的な酵素オリゴヌクレオチド合成のためのナノスケープインターフェースを開発する.
  • EOSのプライマーのアクセシビリティと酵素の空間的な障害を克服する.
  • DNAの情報保存などの用途で DNA合成の収量と精度を高めるため

主な方法:

  • 3D DNAのフレームワークとして四面体DNAナノ構造 (TDN) を利用した.
  • DNAプライマーのオーダーされた指向と間隔を提供する ナノスケールインターフェースを設計した.
  • 酵素と基板の親和性および反応動態に対するTDNの効果を調査した.

主要な成果:

  • TDNの構造は,単一鎖構造と比較して,酵素アクセシビリティと触媒効率を大幅に改善しました.
  • TDNベースのEOSは,パターンのDNA配列の合成中に削除エラーを軽減し,出力を増加させた.
  • ステップワイドの96.82%の収率で60核酸のDNA断片を合成し,15バイトのテキスト情報を正確に取得することができました.

結論:

  • 開発されたTDNベースのナノインターフェースは,高効率で正確な酵素オリゴヌクレオチド合成を可能にします.
  • このアプローチは DNA合成技術の進歩に 堅固な基盤を提供しています
  • アプリケーションには,改良されたDNA情報保存と強化された遺伝子研究機能が含まれます.