オレアノール酸は,胆酸-TGR5/AMPK/mTOR軸経由で小豚の腸細胞増殖とタンパク質合成能力を調節する
Zhenya Zhai1, Weirong Zeng1, Kai-Min Niu1
1Jiangxi Functional Feed Additive Engineering Laboratory, Institute of Biological Resource, Jiangxi Academy of Sciences, Nanchang, Jiangxi 330096, China.
Journal of agricultural and food chemistry
|September 3, 2025
まとめ
オレアノール酸 (OA) のサプリメントは,タケダのGタンパク質結合受容体5 (TGR5) を調節することによって,小豚の胆酸を減少させ,腸の健康を改善しました. これはOAを強調しています.
科学分野:
- 胃腸内科
- 栄養学
- 分子生物学
背景:
- タケダGタンパク質結合受容体5 (TGR5) は,腸の健康における胆酸シグナル伝達に不可欠である.
- 腸機能の調節におけるTGR5の正確なメカニズムは,まだ完全に理解されていません.
研究 の 目的:
- オレアノール酸 (OA) が小豚の胆酸代謝とTGR5シグナル伝達に及ぼす影響を調査する.
- OA媒介による腸の健康調節におけるTGR5/AMPK/mTOR経路の役割を明らかにする.
主な方法:
- 20日間,オレアノール酸 (OA) 50 mg/kgで豚の餌を補給する.
- 血清と腸の胆酸濃度の測定
- 腸細胞におけるTGR5発現とその下流信号経路 (AMPK,mTOR) の評価
- 原蛋白とアミノ酸の表面的消化性と腸内細胞増殖の評価
主要な成果:
- OAサプリメントは血清と腸内全胆酸濃度を大幅に低下させた.
- OAはTGR5発現を抑制し,腸内細胞増殖を促した.
- ハイオコール酸 (HCA) はTGR5/AMPKを活性化させ,mTORを抑制し,細胞増殖とタンパク質合成を抑制した.
- TGR5/AMPK経路の阻害により,OAによる効果が逆転した.
結論:
- オレアノール酸は,小豚の腸の健康を改善するための機能的な飼料添加物としての可能性を示しています.
- 胆汁酸-TGR5軸をターゲットにすることで,腸疾患における栄養介入のための新しい戦略を提示します.
関連する概念動画
01:12Role Of Notch Signalling In Intestinal Stem Cell Renewal
2.2K
Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
2.2K
01:23Renewal of Intestinal Stem Cells
2.7K
The intestinal epithelial lining rapidly renews every 4 to 5 days. The renewal is facilitated by intestinal stem cells (ISCs) located at the base of the crypt– a gland located at the bottom of each villus. ISCs divide asymmetrically to form new stem cells and progenitor daughter cells. The daughter cells are called transit-amplifying (TA) cells which move upwards along the crypt and either differentiate into absorptive cells– the enterocytes or secretory cells– including the...
2.7K
01:16TGF - β Signaling Pathway
7.6K
The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors...
7.6K
01:22PI3K/mTOR/AKT Signaling Pathway
3.9K
The mammalian target of rapamycin (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1 (mTORC1) and mTOR complex 2 (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast, mTORC2 consists of a...
3.9K
02:33Hedgehog Signaling Pathway
7.5K
The Hedgehog gene (Hh) was first discovered due to its control of the growth of disorganized, hair-like bristles phenotype in Drosophila, much like hedgehog spines. Hh plays a crucial role in the development of organs and the maintenance of homeostasis in both invertebrates and vertebrates. However, while Drosophila has only one Hh protein, mammals have multiple functional Hedgehog proteins - Sonic (Shh), Desert (Dhh), and Indian Hedgehog (Ihh). All of these homologous proteins have adapted to...
7.5K