関連する実験動画
Updated: May 30, 2026

25:12
Mechanical Stimulation of Stem Cells Using Cyclic Uniaxial Strain
Published on: July 29, 2007
12.9K
肺疾患のメカニズムを研究するために,Flexcellの張力システムを使用して,アルベオラ上皮質-繊維細胞モデルのダイナミックな機械的刺激
Safiya Al Yazeedi1, Tony Ju Feng Guo1,2, Joban Sohd1
1Department of Biology, University of British Columbia - Okanagan Campus, Kelowna, BC, Canada.
Frontiers in medicine
|September 3, 2025
まとめ
肺モデルにおける機械的なストレスは 細胞数と炎症を増加させます 3次元モデルは肺組織を より良く複製し 線維症のような疾患メカニズムに 洞察力を提供します
科学分野:
- 生物医学工学
- 細胞生物学
- 肺医学
背景:
- 肺の病理生理学は機械的なストレスの影響を受けます.
- 既存の2D in vitroモデルは肺のダイナミックな機械的環境を十分に捉えていません.
- ストレスを誘発した肺の反応を研究するには,高度な多細胞モデルが必要です.
研究 の 目的:
- 機械的に圧迫された2Dと3Dのアルベオラコカルチャーとオルガノイドモデルを開発し,評価する.
- これらのモデルにおける細胞反応に対する機械的ストレスの影響を調査する.
- 肺疾患のメカニズムを研究するためのこれらのモデルの有用性を評価する.
主な方法:
- 人間のA549上皮細胞とMRC-5線維芽細胞を用いた2Dおよび3Dアルベオラ共同培養とオーガノイドを開発した.
- 機械的なストレスを適用するためにFlexcellセルストレッチバイオリアクターを利用しました (18%の幅,24時間0.4Hz).
- 細胞の増殖,形態,細胞骨格および緊密な結合タンパク質の発現,サイトカインの放出 (IL-6,IL-8),および細胞死亡を評価した.
主要な成果:
- 機械的なストレスは3D共同培養で細胞数を大幅に増加させ,増殖を示唆しました.
- 3D培養で生じた線維細胞の形態的変化とF-アクチンの発現の増加.
- ゾヌラオクラデンス-1発現の減少,IL- 6とIL- 8の放出の増加,細胞死亡の増加がモデル全体,特に3D培養で観察されました.
結論:
- Flexcellシステムを用いた機械的にストレートされた in vitro 多細胞膜モデルは, in vivo 肺組織ダイナミクスを真似することができる.
- これらのモデルは,肺炎と線維症に関連するストレスを誘発した細胞反応を調査するための貴重なプラットフォームを提供します.
- これらのモデルは,病変の進行における上皮細胞とメゼンキマの相互作用を調査するのに特に有用である.
関連する概念動画
Mechanism of Breathing I: Inspiration
Introduction to Inspiration: The Respiratory System in Action
The respiratory system, an essential network for breathing, comprises the conducting and respiratory zones, each playing a crucial role in the overall process of respiration. Let us explore the detailed mechanism of inspiration, or inhalation, which is the first phase of the respiratory cycle.
Pathway of Air during Inspiration
During inspiration, air enters our body through the nose or mouth and moves through the conducting zone,...
The respiratory system, an essential network for breathing, comprises the conducting and respiratory zones, each playing a crucial role in the overall process of respiration. Let us explore the detailed mechanism of inspiration, or inhalation, which is the first phase of the respiratory cycle.
Pathway of Air during Inspiration
During inspiration, air enters our body through the nose or mouth and moves through the conducting zone,...
Gross Anatomy of the Lungs
The lungs are a pair of vital organs connected to the trachea via the left and right bronchi. The base of these organs meets the dome-shaped muscle known as the diaphragm. Encased by the pleurae, the lungs contact the mediastinum. The right lung is shorter yet wider, and has a larger volume than the left lung. The left lung has an indentation known as the cardiac notch. The superior region of the lungs is referred to as the apex, whereas the base is the lower region near the diaphragm. The...
Factors Affecting Pulmonary Ventilation
Besides the pressure difference between the external environment and the lungs, the airflow rate and ease of pulmonary ventilation are also influenced by three other factors: surface tension of the fluid in the alveoli, compliance of the lungs, and airway resistance.
Alveolar Surface Tension
The alveolar fluid lines the luminal surface of the alveoli and exerts a force called surface tension. This force is caused by the polar water molecules in the liquid being more strongly attracted to each...
Alveolar Surface Tension
The alveolar fluid lines the luminal surface of the alveoli and exerts a force called surface tension. This force is caused by the polar water molecules in the liquid being more strongly attracted to each...
Mechanical Ventilation II: Invasive Ventilation
Ventilators are essential medical equipment used to aid patients with respiratory difficulties. Their primary function is to assist or replace spontaneous breathing by providing mechanical ventilation. There are two general classes of mechanical ventilators: negative-pressure and positive-pressure ventilators.
Negative-Pressure Ventilators
Negative-pressure ventilators create a vacuum around the chest or body to draw air into the lungs, simulating breathing. This method does not require an...
Negative-Pressure Ventilators
Negative-pressure ventilators create a vacuum around the chest or body to draw air into the lungs, simulating breathing. This method does not require an...
Pneumothorax II: Pathophysiology
Pneumothorax means the presence of air in the pleural space — the thin potential gap between the visceral and parietal pleura. This condition disrupts the normal pressure balance that keeps the lungs inflated, leading to partial or complete collapse of the affected lung.Normal physiologyUnder normal conditions, the pleural space maintains a slightly negative intrapleural pressure, which keeps the lungs expanded against the chest wall. This negative pressure creates a delicate balance between...

