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Updated: Sep 9, 2025

Rapid Verification of Terminators Using the pGR-Blue Plasmid and Golden Gate Assembly
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Rapid Verification of Terminators Using the pGR-Blue Plasmid and Golden Gate Assembly

Published on: April 25, 2016

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植物合成生物学におけるプロモーターとターミネーターの識別と適用

Hyunjin Koo1, Minah Jung1, Sangwoo Lee1

  • 1Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 34141, Republic of Korea.

Molecules and cells
|September 3, 2025
PubMed
まとめ

植物合成生物学では,プロモーターやターミネーターのような新しい生物学的部位を用いて 遺伝子調節を進めている. このレビューでは,医薬品,バイオ燃料,植物天然製品の生産における精密制御の適用について検討しています.

キーワード:
バイオパーツ植物合成生物学プロモーターターミネーターシス規制要素 (CREs)

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Plant Promoter Analysis: Identification and Characterization of Root Nodule Specific Promoter in the Common Bean

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関連する概念動画

The Eukaryotic Promoter Region02:40

The Eukaryotic Promoter Region

The eukaryotic promoter region is a segment of DNA located upstream of a gene. It contains an RNA polymerase binding site, a transcription start site, and several cis-regulatory sequences.  The proximal promoter region is located in the vicinity of the gene and has cis-regulatory sequences and the core promoter. The core promoter is the binding site for RNA polymerase and is usually located between -35 and +35 nucleotides from the transcription start site. The distal promoter regions are...
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Transgenic Plants

Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
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Plant Breeding and Biotechnology

Crop cultivation has a long history in human civilization, with records showing the cultivation of cereal plants beginning at around 8000 BC. This early plant breeding was developed primarily to provide a steady supply of food.
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Conservative Site-specific Recombination and Phase Variation

Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
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Reporter Genes

Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
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Transcription Attenuation in Prokaryotes

Transcriptional attenuation occurs when RNA transcription is prematurely terminated due to the formation of a terminator mRNA hairpin structure.  Bacteria use these hairpins to regulate the transcription process and control the synthesis of several amino acids including histidine, lysine, threonine, and phenylalanine. Transcription attenuation takes place in the non-coding regions of mRNA.
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