関連する実験動画
Updated: Sep 9, 2025

09:16
Meiotic Spindle Assessment in Mouse Oocytes by siRNA-mediated Silencing
Published on: October 11, 2015
11.8K
RNA結合タンパク質SERBP1は,マウスの卵細胞発育と成熟に不可欠である
Lingjuan Wang1, Sijin OuYang2, Bin Li3
1Reproductive Medicine Center, Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School, Nanjing, China.
Molecular and cellular endocrinology
|September 3, 2025
まとめ
SERBP1は女性の生育に不可欠であり,卵細胞の成長と発達を調節する. 卵細胞の成熟と周囲の細胞との通信を妨げることで不妊症を引き起こす.
科学分野:
- 生殖生物学
- 分子生物学
- 遺伝学
背景:
- RNA結合タンパク質 (RBPs) は遺伝子発現の調節に不可欠である.
- SERPINE1 mRNA結合タンパク質1 (SERBP1) は女性の生殖系で顕著に発現しています.
- オオゲネシスと女性の生殖能力におけるSERBP1の役割は完全に理解されていません.
研究 の 目的:
- 哺乳類の卵細胞発育と雌の生育におけるSERBP1の機能を調査する.
- SERBP1が卵細胞の能力と体と胚の相互作用に影響を与えるメカニズムを解明する.
主な方法:
- Zp3/Gdf9-Creドライバを使用した卵細胞におけるSerbp1遺伝子の条件付きノックアウト.
- 卵細胞の成長,生殖能力,早期胚の発達に関する現象分析.
- 粒状細胞のアポプトーシスとシグナル伝達経路 (Erk1/2リン酸化) の評価
主要な成果:
- Serbp1の条件付きの消去は,卵細胞の成長の停止,女性の不妊症,ブラストシストの形成の失敗につながった.
- SERBP1が欠けていた卵細胞は,スパインドル組成の欠陥,不対称な分裂,およびメオティック能力の低下を示した.
- Serbp1の消去は粒状細胞のアポトーシスを誘発し,Erk1/ 2のシグナル伝達が変化し,体内の微環境に影響を与えました.
結論:
- SERBP1は卵子の発達能力と女性の生育能力に欠かせない.
- SERBP1は,細胞自律的メカニズムと体と生殖細胞の交響を通してオオゲネシスを調節する.
- SERBP1の調節不良は卵巣の微小環境に影響を与え,不妊症に寄与する.
関連する概念動画
piRNA - Piwi-interacting RNAs
7.0K
PIWI-interacting RNAs, or piRNAs, are the most abundant short non-coding RNAs. More than 20,000 genes have been found in humans that code for piRNAs while only 2000 genes have been found for miRNAs. piRNAs can act at the transcriptional and post-transcriptional levels and have a vital role in silencing transposable elements present in germ cells. They are also involved in epigenetic silencing and activation. Previously, they were thought to function only in germ cells but new evidence suggests...
7.0K
Transcription Initiation
16.7K
Initiation is the first step of transcription in eukaryotes. Prokaryotic RNA Polymerase (RNAP) can bind to the template DNA and start transcribing. On the other hand, transcription in eukaryotes requires additional proteins, called transcription factors, to first bind to the promoter region in the DNA template. This binding helps recruit the specific RNAP that can assemble on the DNA and start transcription.
The promoters and enhancers and their accessory proteins allow tight regulation of...
The promoters and enhancers and their accessory proteins allow tight regulation of...
16.7K
Ribosomal RNA Synthesis
13.4K
Ribosome synthesis is a highly complex and coordinated process involving more than 200 assembly factors. The synthesis and processing of ribosomal components occurs not only in the nucleolus but also in the nucleoplasm and the cytoplasm of eukaryotic cells.
Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...
Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...
13.4K
Eukaryotic RNA Polymerases
24.6K
RNA Polymerase (RNAP) is conserved in all animals, with bacterial, archaeal, and eukaryotic RNAPs sharing significant sequence, structural, and functional similarities. Among the three eukaryotic RNAPs, RNA Polymerase II is most similar to bacterial RNAP in terms of both structural organization and folding topologies of the enzyme subunits. However, these similarities are not reflected in their mechanism of action.
All three eukaryotic RNAPs require specific transcription factors, of which the...
All three eukaryotic RNAPs require specific transcription factors, of which the...
24.6K
RNA Polymerase II Accessory Proteins
9.4K
Proteins that regulate transcription can do so either via direct contact with RNA Polymerase or through indirect interactions facilitated by adaptors, mediators, histone-modifying proteins, and nucleosome remodelers. Direct interactions to activate transcription is seen in bacteria as well as in some eukaryotic genes. In these cases, upstream activation sequences are adjacent to the promoters, and the activator proteins interact directly with the transcriptional machinery. For example, in...
9.4K
Directing Proteins to the Rough Endoplasmic Reticulum
7.4K
The organelle-specific signaling sequences direct proteins synthesized in the cytosol to their final destination like ER, mitochondria, peroxisomes, etc. Some of the proteins directed to ER are then trafficked via vesicles to other organelles within the cell or the extracellular environment through the Golgi complex. For example, the rough ER synthesizes soluble proteins for transportation to the lysosomes or secretion out of the cell. It can also synthesize transmembrane proteins that can...
7.4K

