OsWRKY113は,Fusarium fujikuroiに対するジャスモニック酸に依存する免疫反応を弱めている
Yujeong Jeong1,2, Ji In Jang1, Eun-Jung Suh1
1National Institute of Agricultural Sciences, Rural Development Administration, Jeonju, 54874, Republic of Korea.
Planta
|September 3, 2025
まとめ
米にあるOsWRKY113遺伝子は,ジャスモニック酸 (JA) のシグナル伝達を抑制することで,フサリウムフジキュロイに対する免疫を否定的に調節する. この発見は,バカナ病に対する米の防御におけるOsWRKY113の新たな役割を明らかにしています.
科学分野:
- 植物病理学
- 分子 植物 微生物 相互作用
- 植物 分子 生物学
背景:
- フサリウム・フジキュロイは,米 (Oryza sativa) のバカナ病を引き起こす.
- WRKY転写因子は,米の病原体との相互作用において極めて重要です.
- F. fujikuroiに対する米の感受性に関与する特定のOsWRKY遺伝子はほとんど特徴づけられていない.
研究 の 目的:
- F. fujikuroiに対する米の免疫におけるOsWRKY113の役割を特定し,特徴づけること.
- 米の防御におけるOsWRKY113の機能の基礎となる分子メカニズムを解明する.
主な方法:
- F.フジキュロイの遺伝子発現分析
- OsWRKY113のサブセルラー局所化
- OsWRKY113を過剰発現するトランスジェニック・ライス・ラインの生成と分析
- ジャスモニック酸 (JA) の生物合成とシグナル伝達経路の評価
- JA反応性病原性 (PR) 関連遺伝子の分析
主要な成果:
- OsWRKY113の発現はF. fujikuroi感染によって誘発され,タンパク質は核に局所する.
- OsWRKY113の構成表現は,JAの生物合成とシグナル伝達に障害をもたらします.
- OsWRKY113の過剰発現は,OsPR9を含むJA反応性PR遺伝子を抑制する.
- 外因的なメチルジャスモナート投与は 感受性の高まったフェノタイプを回復させる.
結論:
- OsWRKY113は,F. fujikuroiに対する米の免疫の負の調節剤として作用する.
- OsWRKY113の機能は,ジャスモニック酸による免疫反応の抑制を伴う.
- この研究は,OsWRKY転写因子家族と米の防御におけるOsWRKY113のこれまで未知の役割を明らかにした.
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