淡水カタツムリの腸内微生物から分離されたLysinibacillus macroides,Kurthia huakuii,およびEnterococcus faeciumのプロバイオティック,抗微生物および抗癌特性
Reham Alaa Eldin Shaker1, Rasha A Hashem2, Mariam Hassan2,3
1Department of Microbiology and Immunology, Faculty of Pharmacy, Cairo University, Kasr Al-Aini, Cairo, 11562, Egypt. riham.gamil@pharma.cu.edu.eg.
BMC biotechnology
|September 3, 2025
まとめ
淡水カタツムリには 多様な腸内細菌があり プロバイオティクスの可能性もあります これらの微生物は抗菌性および抗癌性を持ち,特にKurthia huakuiiは,治療的な応用が可能です.
科学分野:
- 微生物学
- 海洋生物学
- バイオテクノロジー
背景:
- 哺乳類と比べると 軟体系の腸内微生物の理解は限られている.
- 淡水カタツムリの腸内微生物群の 益生菌と抗がん菌株の調査
研究 の 目的:
- 3種類の淡水牛の腸内微生物の構造を記述する.
- 抗菌性および抗がん性を持つ潜在的なプロバイオティック菌株を隔離し評価する.
主な方法:
- 微生物コミュニティの分析のための16S rRNA遺伝子配列化.
- バクテリア菌株 (リシニバシルス・マクロイドス,クルシア・フアキュイ,エンテロコッカス・フェシウム) の分離と識別
- 抗菌薬効性試験,耐性試験 (酸性pH,胆塩),および抗癌能力に対する細胞毒性試験 (MTT,Caco-2細胞)
主要な成果:
- 牛の種によって微生物の豊かさと組成が 大きく異なっています
- プセドモナドタとバクテロイドタが優勢だった.
- 孤立した菌株 (L. macroides,K. huakuii,E. faecium) は,抗菌効果と酸性pHと胆汁塩に対する耐性を示した.
- これらの単離物からの代謝物はCaco-2細胞に対する抗癌活性を示し,K. huakuiiは最高レベルのアポトーシスおよびネクロシス誘導を示した.
結論:
- 淡水カタツムリの腸内微生物群は多様で 特徴的な組成の違いがあります
- 孤立したバクテリア株は 有望な抗菌性および抗癌性を持っています
- カタツムリは生物技術や治療に役立つ新種のバクテリア,特にK. huakuiiの貴重な供給源です.
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