精製されたキュルビタシンDは,MDA- MB-468およびMCF-7ヒト乳がん細胞におけるアポプトシスおよびオートファジック遺伝子発現の変化を引き起こす

Elham Zeinali¹, Seyed Jalal Zargar¹, Najmeh Mozdoori²

¹Department of Cell and Molecular Biology, School of Biology, College of Science, University of Tehran, Tehran, Iran.

Asia-Pacific journal of clinical oncology

|September 3, 2025

まとめ

クキュルビタシンD (CuD) は,乳がん細胞の活性を低下させ,アポトーシスとオートファギーの遺伝子発現を変化させることで,抗がん効果を示す. これらの発見は,CuD

科学分野:

植物化学
分子生物学
腫瘍学

背景:

植物由来化合物は,がん治療のために研究されています.
クキュルビタシンD (CuD) は,信号伝達経路を通じて癌細胞のアポトーシスを誘発する.

研究の目的:

乳がん細胞の生存力に対するCucurbitacin D濃度の変化の影響を調査する.
CuDが細胞死亡パターンと遺伝子発現に与える影響を分析する.

主な方法:

MCF-7およびMDA-MB-468細胞に対する抗増殖効果を評価するためのMTT測定法.
アポトーシス (Bcl-2, Bax,caspase-3,p53) と自己死 (Atg5,Beclin-1,PTEN,Akt) マーカーの遺伝子発現を評価するためのリアルタイムPCR.

主要な成果:

CuDは,両方の細胞系に有意な投与量依存の抗増殖効果を示した (IC50はMCF-7で約30μM,MDA- MB-468では25μM).
CuD治療に反応して,アポトーシスおよびオートファジーに関連する遺伝子の発現の有意な変化が観察されました.

結論:

CuDは特定の用量では乳がん細胞の生存能力と遺伝子発現に影響します.
観察された遺伝子発現の差異は,異なる乳がん亜型の病理生理学と治療反応における異なる役割を示唆している.

キーワード:

アポトーシスオートファジー乳がん細胞の生存能力クキュルビタシンD

さらに関連する動画

11:13

Author Spotlight: Exploring Salidroside's Molecular Mechanisms in Breast Cancer Treatment

Published on: June 9, 2023

1.7K

09:18

Identification of Intracellular Signaling Events Induced in Viable Cells by Interaction with Neighboring Cells Undergoing Apoptotic Cell Death

Published on: December 27, 2016

8.8K

関連する概念動画

01:31

The Intrinsic Apoptotic Pathway

6.8K

Internal cellular stress, such as cellular injury or hypoxia, triggers intrinsic apoptosis. The B-cell lymphoma 2 (Bcl-2) family of proteins are the primary regulators of the intrinsic apoptotic pathway. For example, during DNA damage, checkpoint proteins, such as Ataxia Telangiectasia Mutated (ATM protein) and Checkpoints Factor-2 (Chk2) proteins, are activated. These proteins phosphorylate p53 which further activates pro-apoptotic proteins, such as Bax, Bak, PUMA, and Noxa, and inhibits...

6.8K

02:23

Abnormal Proliferation

4.6K

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...

4.6K

02:34

Inhibition of Cdk Activity

4.9K

The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...

4.9K

01:17

The Extrinsic Apoptotic Pathway

6.6K

The extrinsic apoptotic pathway is initiated when extracellular death-inducing signals, such as specific cytokines, activate the death receptors expressed on the cell surface. The immune cells involved in this pathway are natural killer cells (NK cells) and cytotoxic T-lymphocytes. NK cells are critical in innate immune response, while cytotoxic T-lymphocytes are associated with adaptive immune response. These cells recognize specific receptors expressed on the altered cells and activate...

6.6K

01:18

Autophagic Cell Death

3.5K

Christian de Duve discovered “autophagy,” a process in which cellular components are engulfed by membrane-bound organelles called autophagosomes. The autophagosomes then fuse with lysosomes to digest the enclosed contents. Autophagy is generally activated in cells to prevent cell death. However, cell death is triggered when the damage is beyond repair.
Autophagy and Apoptosis
Autophagy can activate apoptosis. In normal conditions, the autophagy activating protein Beclin-1 and...