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自然に感染したブロッコリの種からAlternaria brassicicolaの2つのサクシナート脱水素酶阻害菌に対するクロスレジスタンス
Navjot Kaur1, Anoop A Malik1, Daniel G Cerritos-Garcia2
1Department of Plant Pathology, University of Georgia, Tifton, Georgia, USA.
Applied and environmental microbiology
|September 4, 2025
まとめ
ブロッコリの種に含まれる病原体Alternaria brassicicolaは,スッキナート脱水素酵素阻害剤 (SDHI) に対する耐性を示しています. この抵抗性は特定の遺伝子変異と関連しており 種子による病気の拡散の危険性を強調しています
科学分野:
- 植物病理学
- 菌類の遺伝学
- 農業科学
背景:
- Alternaria brassicicolaのような真菌病原体は 商業的な種子を通して 世界中に拡散できます
- 以前の研究で,アゾキシストロビンに耐性のある Alternaria brassicicola がブロッコリの種に検出されました.
- 植物病原体の真菌駆除剤耐性は作物生産に重大な脅威をもたらす.
研究 の 目的:
- ブロッコリの種から分離したAlternaria brassicicolaのサクシネート脱水素酵素阻害剤 (SDHI) に対する感受性を調べる.
- これらの単離体におけるSDHI耐性の遺伝的基礎を特定する.
- Alternaria brassicicolaのSDHI耐性に対する迅速な検出方法を開発する.
主な方法:
- ブロッコリの種子から分離した58種のAlternaria brassicicolaの真菌殺菌剤のインビトロ評価
- 菌類殺菌剤耐性に関連する変異を特定するためにSDH遺伝子の配列分析.
- SDHCにおけるH134R変異を検出するためのアレル特異のプライマーの開発と検証.
主要な成果:
- 単離物の93%はボスカリドとペンチオピラド (SDHI菌殺剤) に耐性であった.
- SDHI耐性とは,SDHC遺伝子の単点変異 (H134R) が関連していた.
- SDHI耐性の迅速な検出のためのPCR検査を開発し,耐性単離体が競争力のある成長を維持した.
結論:
- この研究は,商用ブロッコリの種から得たAlternaria brassicicolaのSDHI菌殺剤耐性の最初の証拠を提供します.
- ブロッコリの種は,真菌剤に耐性のある真菌病原体を拡散させる媒介として機能する.
- 種子の健康検査と真菌駆除剤耐性のモニタリングは 効果的な病気管理に不可欠です
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