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RoAM:古代メチロームの計算再構築と異なったメチル化領域の識別

Yoav Mathov^1,2, Naomi Rosen¹, Chen Leibson¹

¹Department of Genetics, The Alexander Silberman Institute of Life Sciences, Faculty of Science, the Hebrew University of Jerusalem, Jerusalem, 9190401, Israel.

|September 4, 2025

まとめ

古代DNAメチル化分析のための RoAM (古代メチル化再構築) ソフトウェアを改良しました私たちの研究は糖質代謝の遺伝子とインスリン調節に影響する食生活の変化を明らかにしていますバルカン半島の新石器時代の移行後です

科学分野:

パレオゲノミクス
エピジェネティクス
古代の DNA の分析

背景:

古代のDNAメチル化 (表遺伝学) は過去の生物学的過程の洞察を提供します.
古代メチロームの再構築は進化と歴史的変化を理解するために不可欠です
以前のツールには,包括的な古伝承学研究のための柔軟性と信頼性が欠けていました.

研究の目的:

RoAM (リコンストラクション・オブ・アンス・メチレーション) ソフトウェアの改良版を導入する.
古代メチロームの頑丈な再構築と微分メチル化領域 (DMR) の識別を可能にします.
バルカン諸島の人口における新石器時代の移行に関連した表遺伝的変化を調査する.

主な方法:

洗練された RoAM パイプラインを使用し,高度なフィルタリングと品質管理を行います.
改良されたRoAMツールを新石器時代前後のバルカン人の古代DNAサンプルに適用する.
関連する遺伝子のDMRを特定するために微分メチル化分析を行う.

主要な成果:

強化されたRoAMツールは高品質の古代DNAメチル化マップを生成しました
新石器時代以前のサンプルと新石器時代後のサンプルで糖質代謝に関連する遺伝子の有意なDMRを特定した.
インスリン分泌を調節する遺伝子であるPTPRN2の新石器時代後の超メチル化が観察された.

結論:

改良されたRoAMは古伝承学にとって強力なツールです
新石器時代における食生活の変化は代謝の表遺伝的調節に影響した.
新石器時代以前の狩猟採集者の集団における潜在的低インスリン症候群を示唆しています

キーワード:

古代のDNA 古代のDNAメチル化食事のシフト人類の進化新石器時代の移行期

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