神経成長因子を封じ込めるコアシアル・エレクトロスポン・アラインド・コア・シェル・ポリ (エチレン酸化物-ポリ ((l-ラクチド-コグリコリド)) ナノファイバーのプロセスの最適化と放出モデリング
Bhoomija Hariprasad1, Mohammadjavad Eslamian1, Nihir Patel1
1Department of Biomedical Engineering, University of Houston.
まとめ
この研究では,同軸の電気回転を最適化して,神経再生のための並列神経成長因子 (NGF) 負荷ナノファイバーを作成しました. このプロセスは最小の繊維直径と狭いサイズ分布を達成し,制御されたタンパク質の放出を可能にしました.
科学分野:
- バイオマテリアル科学
- 神経工学
- ポリマー化学
背景:
- 神経再生戦略は,ポリマーカプセル内のタンパク質によって提供される地形学的および生化学的なシグナルから恩恵を受けます.
- コアシアル・エレクトロスピニングは,制御された放出のために,生物活性物質をコアシェルナノファイバーに埋め込むための重要な技術です.
研究 の 目的:
- 神経成長因子 (NGF) を負荷した並列されたポリエチレン酸化物) -ポリエチレン酸化物) ナノファイバーを最適化するための同軸電気回転パラメータを体系的に調査する.
- 繊維直径の最小化と狭いサイズ分布を実現し,神経再生のアプリケーションを強化します.
主な方法:
- 最適な電気回転パラメータを特定するために,実験の設計方法としてBox-Behnken Design (BBD) を利用した.
- 流量,コレクター速度,電圧,距離などのパラメータがナノファイバーの特性に及ぼす影響を決定するために回帰分析を行った.
- 最適化されたコアシェルナノファイバーを使用してNGF放出運動を特徴づけた.
主要な成果:
- 繊維直径 (323 nm) とサイズ分布 (2.37%) は,特定のパラメータ設定で達成されました:内流率 (0.33 mL/h),外流率 (2 mL/h),コレクター速度 (500 rpm),電圧 (17 kV),距離 (10 cm).
- 内流量,コレクター距離,および電圧は繊維直径に大きく影響し,コレクター速度はサイズ分布に決定的でした.
- NGFの放出は二段階的行動を示した:最初の爆発的な放出 (約81% 8時間),続いて持続的な放出 (約13% 2週間),ミカエリス-メンテンモデルに適合する.
結論:
- 制御された寸法でNGFを装着したコアシェルのナノファイバーを製造するための効率的で持続可能な方法が確立されました.
- 最適化されたナノファイバーは 精密な地形学および生化学的ヒントを提供することで 神経再生療法を進歩させる大きな可能性を秘めています
- 双相放出運動は,PEOコア溶解とPLGA分解メカニズムを示唆し,調節可能な薬物投与プロファイルを提供します.
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