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関連する概念動画

CRISPR and crRNAs02:53

CRISPR and crRNAs

17.3K
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
17.3K
The Antiviral System of Bacteria and Archaea: CRISPR01:23

The Antiviral System of Bacteria and Archaea: CRISPR

121
CRISPR stands for Clustered Regularly Interspaced Short Palindromic Repeats is a adaptive immune system found in bacteria and archaea that protects against viral infections. This system enables prokaryotic cells to identify, remember, and neutralize foreign genetic elements, primarily bacteriophages, by storing fragments of the invader’s DNA as a genetic memory.The CRISPR immune response begins during an initial infection. Cas (CRISPR-associated) proteins play a central role in this...
121
CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

208
The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
208
CRISPR01:59

CRISPR

52.8K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
52.8K
Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

6.1K
Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
6.1K

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Updated: Sep 9, 2025

Application of CRISPR Interference CRISPRi for Gene Silencing in Pathogenic Species of Leptospira
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マルチロカス配列型17株におけるCRISPR干渉

William D Cutts, Aidan W Flanagan, Brice Gorman

    bioRxiv : the preprint server for biology
    |September 5, 2025
    PubMed
    まとめ

    研究者らは,グループBのストレプトコッカス (GBS) ST-17菌株に対するCRISPR干渉システムを開発した. このツールは,血脳障壁のGBS髄膜炎の病原性を研究するために,標的の遺伝子ノックダウンを可能にします.

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    Removal of an Internal Translational Start Site from mRNA While Retaining Expression of the Full-Length Protein
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    Removal of an Internal Translational Start Site from mRNA While Retaining Expression of the Full-Length Protein

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    関連する実験動画

    Last Updated: Sep 9, 2025

    Application of CRISPR Interference CRISPRi for Gene Silencing in Pathogenic Species of Leptospira
    14:49

    Application of CRISPR Interference CRISPRi for Gene Silencing in Pathogenic Species of Leptospira

    Published on: August 14, 2021

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    Gene Digital Circuits Based on CRISPR-Cas Systems and Anti-CRISPR Proteins
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    科学分野:

    • 微生物学
    • 遺伝学
    • 感染症

    背景:

    • 新生児の細菌性髄膜炎の主な原因である.
    • COH1と同様に,ハイパーウイルス性セロタイプIII,シーケンスのタイプ17 (ST-17) のGBS株は重症の新生児疾患と強く関連しています.
    • ST-17 GBS株の遺伝子操作は困難で,毒性の要因の研究を妨げています.

    研究 の 目的:

    • ST-17 GBS COH1株における標的遺伝子ノックダウンのためのCRISPR干渉 (CRISPRi) システムを開発する.
    • 機能的ゲノミクスとGBSの毒性因子の高通量スクリーニングを可能にします.
    • 血脳障壁のGBS病原性に関する研究を容易にするため

    主な方法:

    • COH1 GBS株で触媒的に不活性化されたCas9 (dCas9) を使用したCRISPR干渉システム (CRISPRi) の開発.
    • ヘモリシスアッセイ,qPCR,ヒト脳内皮細胞によるin vitro感染モデルを用いてシステムの有効性を確認した.
    • pilA,srr2,iagAを含む主要な毒性遺伝子の標的を絞ったノックダウン

    主要な成果:

    • 調節可能なCRISPRiシステムをST-17 GBS COH1で成功裏に実装しました.
    • 本質的なGBSの毒性遺伝子の 顕象的ノックダウンが示された.
    • バクテリアの粘着,侵入,および血脳障壁の炎症反応が減少しました.

    結論:

    • 開発されたCRISPRiシステムは,ST-17 GBSにおける迅速な遺伝子ノックダウンのための汎用的なプラットフォームを提供します.
    • このツールは COH1における以前の遺伝子操作の課題を克服しています
    • 血脳障壁におけるGBSの病原性と宿主-病原体の相互作用に関する先進的な研究を可能にする.