関連する実験動画

Updated: Sep 8, 2025

Chromosome Replicating Timing Combined with Fluorescent In situ Hybridization
17:14

Chromosome Replicating Timing Combined with Fluorescent In situ Hybridization

Published on: December 10, 2012

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クリック化学ベースのバイオチニレーションを用いた全ゲノムDNA複製タイミング分析のためのプロトコル

Deniz Gökbuget1, Kayla Lenshoek1, Robert Blelloch1

  • 1The Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, Center for Reproductive Sciences, University of California, San Francisco, San Francisco, CA, USA; Department of Urology, University of California, San Francisco, San Francisco, CA, USA; Helen Diller Family Comprehensive Cancer Center, University of California, San Francisco, San Francisco, CA, USA.

STAR protocols
|September 5, 2025
PubMed
まとめ

増殖する細胞の全ゲノムにわたる DNA複製のタイミングを マップする新しい方法である BioRepli-seq を開発しました このテクニックは,精密なRTプロファイリングのためにバイオチニレーションと次世代シーケンシングを使用します.

キーワード:
細胞生物学遺伝学ゲノミクス分子生物学

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Lagging Strand Synthesis01:59

Lagging Strand Synthesis

During replication, the complementary strands in double-stranded DNA are synthesized at different rates. Replication first begins on the leading strand. Replication starts later, occurs more slowly, and proceeds discontinuously on the lagging strand.
There are several major differences between synthesis of the leading strand and synthesis of the lagging strand. 1) Leading strand synthesis happens in the direction of replication fork opening, whereas lagging strand synthesis happens in the...
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Chromosome Replication

Before a cell can divide, it must accurately replicate all of its chromosomes, including the DNA and its associated histone and non-histone proteins.  This process begins at numerous origins of replication during the S phase of the cell cycle in each of a cell’s chromosomes simultaneously. Certain nucleotides can act as origins of replication, but these sequences are not well defined - especially in complex, multi-cellular, eukaryotic species. The length of DNA that spans an origin...
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