超高速液体クロマトグラフィーとフライト質量スペクトロメトリーによる炭水化物の定量測定
Anuradha Singh1, Andrew Thompson1, Shyam L Kandel1
1Edward T. Schafer Agricultural Research Center, USDA ARS, Fargo, ND, USA.
まとめ
新しい超高速液体染色法により 砂糖ビートの分析が改善されました この繊細な技術は 砂糖のような炭水化物を正確に定量化し 工業用途の砂糖の収穫率の評価を 改善します
科学分野:
- 農業化学
- 分析化学
- 生物化学
背景:
- 糖ビートの炭水化物の正確な定量化は,糖生産の収穫を最適化するために不可欠です.
- 炭水化物分析の伝統的な方法は,工業加工に必要な感度と速度を欠くことがあります.
研究 の 目的:
- 高感度で選択的な超高速液体クロマトグラフィーと飛行時間質量測定法 (UFLC-ToFMS) の開発と検証
- 砂糖の根の単糖,砂糖,ラフィノースを含む主要な炭水化物の同時分析を可能にします.
主な方法:
- 炭水化物の分析のためのUFLC-ToFMSの方法の開発と検証
- UFLC-ToFMSメソッドの感度および定量化限界 (LOD/LOQ) と,標準的な高性能液体染色学と屈折指数検出 (HPLC-RI) の比較
- 炭水化物の含有量を実際に採取した砂糖ビートの根のサンプルで測定する.
主要な成果:
- UFLC-ToFMS方法は,HPLC-RIと比較して,最大1000倍まで,著しく高い感度を示した.
- 検出限界 (LOD) と定量化限界 (LOQ) はそれぞれ0.03-0.23μg/mLと0.09-0.7μg/mLである.
- 砂糖ビートの根の炭水化物を定量化するための方法を成功裏に適用し,その実用性を確認しました.
結論:
- 開発されたUFLC-ToFMS方法は,砂糖ビートの炭水化物分析に迅速で敏感で選択的なアプローチを提供します.
- この高度な分析技術は,砂糖の収穫量の評価のための従来の方法よりも大幅に改善されています.
- この方法の高い感度と精度により,砂糖加工産業の常用分析に適しています.
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