関連する実験動画

Updated: Sep 8, 2025

A Rapid Screening Workflow to Identify Potential Combination Therapy for GBM using Patient-Derived Glioma Stem Cells
05:29

A Rapid Screening Workflow to Identify Potential Combination Therapy for GBM using Patient-Derived Glioma Stem Cells

Published on: March 28, 2021

2.9K

テモゾロミドによる変異過程の生化学的再構成

Mahima R Sanyal1, Tomohiko Sugiyama1

  • 1Department of Biological Sciences, Ohio University, Athens, Ohio, United States of America; Molecular and Cellular Biology Graduate Program, Ohio University, Athens, Ohio, United States of America.

The Journal of biological chemistry
|September 6, 2025
PubMed
まとめ

テモゾロミド (TMZ) の化学療法は,脳腫瘍における特定のDNA変異 (SBS11) を引き起こします. この研究は,DNA修復酵素MGMTとポリメラーゼデルタが,これらのTMZ誘発変異の形成の鍵であることを明らかにしています.

キーワード:
DNAメチル化DNAポリメラーゼDNA 修復メチルグアニンがんの変異シグネチャーインビトロ変異メチルグアニンメチルトランスフェラーゼテモゾロミド

さらに関連する動画

Method for Novel Anti-Cancer Drug Development using Tumor Explants of Surgical Specimens09:26

Method for Novel Anti-Cancer Drug Development using Tumor Explants of Surgical Specimens

Published on: July 29, 2011

16.8K
Evaluating In Vitro DNA Damage Using Comet Assay10:43

Evaluating In Vitro DNA Damage Using Comet Assay

Published on: October 11, 2017

38.1K

関連する概念動画

Spontaneous and Induced Mutations01:30

Spontaneous and Induced Mutations

Spontaneous mutations arise infrequently during DNA replication due to errors in the process. A key factor behind these errors is tautomeric shifts in nitrogenous bases, where bases transition from keto to enol forms or amino to imino forms. This shift can alter base-pairing rules, leading to mutations. Additionally, reactive oxygen species (ROS) arising from aerobic metabolism can damage DNA, resulting in depurination (loss of a purine base) or depyrimidination (loss of a pyrimidine base).
140
Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
6.1K
In-vitro Mutagenesis01:16

In-vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.
14.2K
Mismatch Repair01:36

Mismatch Repair

Overview
40.6K