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関連する概念動画

Mechanisms of Membrane Domain Formation00:59

Mechanisms of Membrane Domain Formation

3.1K
Different physical properties of lipids and proteins allow them to localize and form distinct islands or domains in the membrane. Some membrane domains are formed due to protein-protein interactions, whereas others are formed due to the presence of specific lipids such as sphingolipids and sterols—for example, large proteins, such as bacteriorhodopsin, aggregate and create distinct domains.
Another mechanism for membrane domain formation involves membrane proteins interacting with...
3.1K
Intracellular Signaling Affects Focal Adhesions01:17

Intracellular Signaling Affects Focal Adhesions

2.8K
Integrins act both as extracellular input receivers and as intracellular processing activators. As their name suggests, integrins are entirely integrated into the membrane structure. Their hydrophobic membrane-spanning regions interact with the phospholipid bilayer's hydrophobic region. These membrane receptors provide extracellular attachment sites for effectors like hormones and growth factors. They activate intracellular response cascades when their effectors are bound and active.
Some...
2.8K
Mechanism of Filopodia Formation01:39

Mechanism of Filopodia Formation

2.5K
Filopodia are thin, actin-rich cellular protrusions that play an important role in many fundamental cellular functions. They vary in their occurrence, length, and positioning in different cell types, suggesting their diverse roles.
Their main function is to guide migrating cells during normal tissue morphogenesis or cancer metastasis by recognizing and making initial contacts with the extracellular matrix. However, they can also act as stationary cell anchors or help to establish communication...
2.5K
Cytoskeletal Linker Proteins - Plakins01:09

Cytoskeletal Linker Proteins - Plakins

2.4K
Plakins are large proteins with binding domains for microtubules, microfilaments, intermediate filaments, and membrane-associated protein complexes at cell junctions. Plakin functions are evolutionarily conserved and are primarily involved in organizing the different components of the cytoskeleton by crosslinking them to each other and connecting them to the cell-matrix and cell adhesion complexes. They are also known to interact with signal transducers, serve as scaffolds for signaling...
2.4K
Lipids as Anchors01:32

Lipids as Anchors

5.8K
In the plasma membrane, the lipids forming the bilayer can also act as an anchor to tether proteins to the membrane. The three main types of lipid anchors found in eukaryotes are – prenyl groups, fatty acyl groups, and glycosylphosphatidylinositol or GPI groups. Prenyl and fatty acyl groups act as anchors on the cytosolic surface of the membrane, whereas GPI anchors proteins on the extracellular side.
The carboxy-terminal of most of the prenylated proteins, such as Ras proteins, contains...
5.8K
Assembly of Signaling Complexes01:30

Assembly of Signaling Complexes

5.9K
Multiprotein signaling complexes are formed in a dynamic process involving protein-protein interactions at the cytoplasmic domain of transmembrane receptors or enzymatic and non-enzymatic proteins associated with the receptor. These complexes ensure the activation and propagation of intracellular signals that regulate cell functions.
Interaction domains in cell signaling
Interaction domains recognize exposed features of their binding partners containing post-translationally modified sequences,...
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関連する実験動画

Updated: Sep 8, 2025

SorLA and CLC:CLF-1-dependent Downregulation of CNTFRα as Demonstrated by Western Blotting, Inhibition of Lysosomal Enzymes, and Immunocytochemistry
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SorLA and CLC:CLF-1-dependent Downregulation of CNTFRα as Demonstrated by Western Blotting, Inhibition of Lysosomal Enzymes, and Immunocytochemistry

Published on: January 6, 2017

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不規則なフェリンC2A-C2Bリンク器 膜を結合し,小さな線形モチーフをエンコードする

Ethiene Kwok1, Patricia Khuu1, Erin Huang1

  • 1Department of Biochemistry and Biophysics Oregon State University, Corvallis, Oregon, USA.

Journal of molecular biology
|September 6, 2025
PubMed
まとめ

フェルリンタンパク質リンクは受動的な距離隔離器ではありません. 信号発信プラットフォームや 結合膜や タンパク質パートナーとして機能し 膀の移動や 細胞機能に不可欠です

キーワード:
IDR についてスリム乱れたエンドサイトーシスリンカー膜

さらに関連する動画

Transmembrane Domain Oligomerization Propensity determined by ToxR Assay
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Transmembrane Domain Oligomerization Propensity determined by ToxR Assay

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Characterization of Proteins by Size-Exclusion Chromatography Coupled to Multi-Angle Light Scattering SEC-MALS
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Characterization of Proteins by Size-Exclusion Chromatography Coupled to Multi-Angle Light Scattering SEC-MALS

Published on: June 20, 2019

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関連する実験動画

Last Updated: Sep 8, 2025

SorLA and CLC:CLF-1-dependent Downregulation of CNTFRα as Demonstrated by Western Blotting, Inhibition of Lysosomal Enzymes, and Immunocytochemistry
10:16

SorLA and CLC:CLF-1-dependent Downregulation of CNTFRα as Demonstrated by Western Blotting, Inhibition of Lysosomal Enzymes, and Immunocytochemistry

Published on: January 6, 2017

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Transmembrane Domain Oligomerization Propensity determined by ToxR Assay
06:45

Transmembrane Domain Oligomerization Propensity determined by ToxR Assay

Published on: May 26, 2011

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Characterization of Proteins by Size-Exclusion Chromatography Coupled to Multi-Angle Light Scattering SEC-MALS
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Characterization of Proteins by Size-Exclusion Chromatography Coupled to Multi-Angle Light Scattering SEC-MALS

Published on: June 20, 2019

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科学分野:

  • 分子生物学
  • 細胞生物学
  • 生物化学

背景:

  • フェルリンは,C2ドメインと不規則なリンク器を特徴とする主要な膀取引タンパク質です.
  • カルシウムセンサーとしてのフェリンC2ドメインの機能は知られているが,リンク器の役割は不明である.
  • C2A-C2Bリンクの役割の調査は,受動的な距離を超えて不可欠です.

研究 の 目的:

  • 脊椎動物のフェルリンC2A-C2B結合体の機能的重要性を調査する.
  • これらの結合体内の潜在的な膜結合モチーフとタンパク質相互作用部位を特定する.
  • リンク機能における代替スプライシングの役割を理解する.

主な方法:

  • 短線形モチーフ (SLiM) と膜結合領域を特定するための配列分析.
  • リポソーム結合測定は,再結合フェルリンC2A-C2B構造を用いる.
  • タンパク質とタンパク質の相互作用を検出するための光スペクトロスコーピー.

主要な成果:

  • フェルリンリンクは,SLiMと膜結合配列,特にオトフェルリンとディスフェルリンを含んでいる.
  • オトフェリンC2A- C2Bリンクは膜結合を有意に媒介し,代替スプライシングの影響を受けます.
  • ディスフェリンリンカーはリポソームと結合し,SH3/WWドメインのタンパク質と相互作用する.

結論:

  • 脊椎動物のフェルリンC2A-C2Bは,シグナリングプラットフォームとして機能し,SLiM結合パートナーを募集します.
  • 特定のリンカー領域は膜結合"ホットスポット"として作用し,タンパク質複合体を潜在的に局所化する.
  • リンカー配列は,膜輸送と細胞シグナル伝達におけるフェルリン機能に不可欠である.