Jove
Visualize
お問い合わせ
JoVE
x logofacebook logolinkedin logoyoutube logo
JoVEについて
概要リーダーシップブログJoVEヘルプセンター
著者向け
出版プロセス編集委員会範囲と方針査読よくある質問投稿
図書館員向け
推薦の声購読アクセスリソース図書館諮問委員会よくある質問
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experimentsアーカイブ
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教員リソースセンター教員サイト
利用規約
プライバシーポリシー
ポリシー

関連する概念動画

Cohesins02:20

Cohesins

5.5K
Cohesin protein complexes are a molecular glue that holds two sister chromatids together. They play an important role both in mitosis and meiosis. In mitosis, all cohesin complexes present on the chromosomes are removed before the start of the anaphase stage.
Cohesin complexes in Meiotic Division
Meiosis involves two distinct rounds of chromosomal segregation and cell divisions— Meiosis I followed by Meiosis II – producing four daughter cells. Meiosis I includes the separation of...
5.5K
The Replisome03:01

The Replisome

38.1K
DNA replication is carried out by a large complex of proteins that act in a coordinated matter to achieve high-fidelity DNA replication. Together this complex is known as the DNA replication machinery or the replisome.
The synthesis of the leading and lagging strands is a highly coordinated process. To explain this, the “Trombone model” was proposed by Bruce Alberts in 1980. The DNA loop formation starts when a primer is synthesized on the parent lagging strand. The loop grows with...
38.1K
The Replisome03:01

The Replisome

9.8K
9.8K
Separation of Sister Chromatids02:17

Separation of Sister Chromatids

4.4K
At the transition from prophase to metaphase, there is a reduction in cohesion along the chromosomal arms, resulting in the resolution of sister chromatids. However, residual cohesin connections remain to hold the sister chromatids together until the transition from metaphase to anaphase. The residual connection prevents any premature separation of sister chromatids, blocking the risks of aneuploidy within the daughter cells.
At the onset of anaphase, separase, a proteolytic enzyme, is...
4.4K
Restarting Stalled Replication Forks02:37

Restarting Stalled Replication Forks

6.3K
DNA replication is initiated at sites containing predefined DNA sequences known as origins of replication. DNA is unwound at these sites by the minichromosome maintenance (MCM) helicase and other factors such as Cdc45 and the associated GINS complex.The unwound single strands are protected by replication protein A (RPA) until DNA polymerase starts synthesizing DNA at the 5’ end of the strand in the same direction as the replication fork. To prevent the replication fork from falling apart,...
6.3K
Restarting Stalled Replication Forks02:37

Restarting Stalled Replication Forks

2.3K
2.3K

こちらも読む

関連記事

共著者、ジャーナル、引用グラフによってこの研究に関連する記事。

並び替え
Same author

Structure of the pre-initiation complex explains CMGE biogenesis.

Nature·2026
Same author

Targeting cancer-specific mutations with RNA-triggered chromatin shredding.

Nature·2026
Same author

Selective Elimination of TP53 Mutant Cells by Transcript-Activated Chromatin Shredding.

bioRxiv : the preprint server for biology·2026
Same author

Investigations into multiple fission yeast chromosome size determinants.

Journal of cell science·2026
Same author

Why is your separase such a big protease?

Science advances·2025
Same author

Biochemical Reconstitution of Replication-Coupled Cohesin Acetylation.

Methods in molecular biology (Clifton, N.J.)·2025

関連する実験動画

Updated: Jan 17, 2026

Author Spotlight: Investigating the Motion Dynamics of the Eukaryotic Replisome Components at the Single-Molecule Level
10:11

Author Spotlight: Investigating the Motion Dynamics of the Eukaryotic Replisome Components at the Single-Molecule Level

Published on: July 26, 2024

1.6K

コヘシン・リングを通る複素の経路

Samson Glaser1, Maxim I Molodtsov2, John F X Diffley3

  • 1Chromosome Replication Laboratory, The Francis Crick Institute, London NW1 1AT, UK; Chromosome Segregation Laboratory, The Francis Crick Institute, London NW1 1AT, UK.

Cell
|September 17, 2025
PubMed
まとめ

ユカリオットの複製ヘリケーズ (CMG) はコヘシンリングを通り抜け,複製されたDNAを捕まえる. この発見は DNA複製と染色体分離の間の直接的なリンクを明らかにし 細胞分裂に影響を与えています

キーワード:
DNA複製染色体分離結束性単分子光顕微鏡姉妹染色体連結

さらに関連する動画

Examination of Mitotic and Meiotic Fission Yeast Nuclear Dynamics by Fluorescence Live-cell Microscopy
12:04

Examination of Mitotic and Meiotic Fission Yeast Nuclear Dynamics by Fluorescence Live-cell Microscopy

Published on: June 24, 2019

10.6K
Detection of Homologous Recombination Intermediates via Proximity Ligation and Quantitative PCR in Saccharomyces cerevisiae
07:55

Detection of Homologous Recombination Intermediates via Proximity Ligation and Quantitative PCR in Saccharomyces cerevisiae

Published on: September 11, 2022

2.2K

関連する実験動画

Last Updated: Jan 17, 2026

Author Spotlight: Investigating the Motion Dynamics of the Eukaryotic Replisome Components at the Single-Molecule Level
10:11

Author Spotlight: Investigating the Motion Dynamics of the Eukaryotic Replisome Components at the Single-Molecule Level

Published on: July 26, 2024

1.6K
Examination of Mitotic and Meiotic Fission Yeast Nuclear Dynamics by Fluorescence Live-cell Microscopy
12:04

Examination of Mitotic and Meiotic Fission Yeast Nuclear Dynamics by Fluorescence Live-cell Microscopy

Published on: June 24, 2019

10.6K
Detection of Homologous Recombination Intermediates via Proximity Ligation and Quantitative PCR in Saccharomyces cerevisiae
07:55

Detection of Homologous Recombination Intermediates via Proximity Ligation and Quantitative PCR in Saccharomyces cerevisiae

Published on: September 11, 2022

2.2K

科学分野:

  • 分子生物学
  • 細胞生物学
  • 遺伝学

背景:

  • コヘシン複合体は,複製後の姉妹染色体を取り囲み,細胞分裂中に正確な染色体分離を保証する.
  • 複製機構がコヘシンと相互作用するメカニズム,特に複製体がコヘシン環を通過できるかどうかについては,特徴づけられていなかった.

研究 の 目的:

  • 直接視覚化し,真核複製ヘリコース (CMG) とコヘシンリングの相互作用を調査する.
  • DNA複製中に複素体がコヘシン環を 通過できるかどうかを判断する.
  • コヘシンを通過する複素体を通過するDNAポリメラーゼの役割を明らかにする.

主な方法:

  • DNA複製機構とコヘシン複合体の生化学的再構成
  • 単分子光顕微鏡を用いて,レプリソーム-コヘシンとの接触をリアルタイムで観察する.

主要な成果:

  • 転位する真核複製ヘリカーゼ (Cdc45-Mcm2-7-GINSまたはCMG) はコヘシン環を通過する.
  • 完全に再構成された複素体はコヘシン環を通り抜け,新たに合成されたDNA鎖の両方を閉じ込めます.
  • DNAポリメラーゼ α と ε は,コヘシンリングを通過するレプリソームを助ける上で重要な役割を果たします.

結論:

  • コヘシン環を通過するレプリソームの通過は,ゲノム複製と染色体分離を結びつける重要なメカニズムである.
  • この発見は,このプロセスにおける統合体制の要素の役割に関する以前の仮定に異議を唱える.
  • この研究は,コヘシン内の複製産物の共同捕獲を保証する安全メカニズムの直接的な証拠を提供します.