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Updated: Jan 17, 2026

Combining Non-reducing SDS-PAGE Analysis and Chemical Crosslinking to Detect Multimeric Complexes Stabilized by Disulfide Linkages in Mammalian Cells in Culture
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Combining Non-reducing SDS-PAGE Analysis and Chemical Crosslinking to Detect Multimeric Complexes Stabilized by Disulfide Linkages in Mammalian Cells in Culture

Published on: May 2, 2019

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タンパク質の構造を検知するためのシステインアンポリング

Philipp Hartmann1,2, Kostiantyn Bohdan1,2, Lara Vogelsang3

  • 1Max-Planck-Institut für Kohlenforschung, Kaiser-Wilhelm-Platz 1, 45470 Mülheim an der Ruhr, Germany.

Journal of the American Chemical Society
|September 19, 2025
PubMed
まとめ

ビニルチアントレニウムテトラフローロボラート (VTT) は,本来のシステニルチオールから電離性エピスルフォニウムイオンを素早く形成することによって,単段階の細胞内タンパク質クロスリンクを可能にします. この方法は,構造の予測のために細胞内タンパク質に効率的にラベルを貼る.

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