関連する実験動画

Updated: Jan 15, 2026

Paramagnetic Relaxation Enhancement for Detecting and Characterizing Self-Associations of Intrinsically Disordered Proteins

Paramagnetic Relaxation Enhancement for Detecting and Characterizing Self-Associations of Intrinsically Disordered Proteins

Published on: September 23, 2021

タンパク質NMRスペクトロシーにおける効率的な偽接触シフト測定のための光駆動パラマグネットタグ

Zhaofei Chai^1,2, Qiong Wu¹, Kai Cheng¹

¹State Key Laboratory of Magnetic Resonance Spectroscopy and Imaging, National Center for Magnetic Resonance in Wuhan, Wuhan National Laboratory for Optoelectronics, Wuhan Institute of Physics and Mathematics, Innovation Academy for Precision Measurement Science and Technology, Chinese Academy of Sciences, Wuhan 430071, China.

Journal of the American Chemical Society

|October 15, 2025

まとめ

この研究では,タンパク質構造の決定のために2組の擬似接触シフト (PCS) を生成するための新しいアゾベンゼンベースのプローブを導入します. この照明制御法では,構造的な制約を単純化し,コストと時間を削減します.

科学分野:

生物化学
構造生物学
化学物理学

背景:

偽接触シフト (PCS) は,タンパク質の構造とダイナミクスの決定に不可欠です.
現在の方法はしばしば複数のタグまたはラベリングを必要とし,複雑さと費用を増加させます.

研究の目的:

単一の,切り替え可能なパラマグネティックプローブを使用してPCSを取得するためのより効率的な方法を開発する.
タンパク質構造の決定に伴う時間と費用を削減する.

主な方法:

フォトイソメリゼーションを可能にするアゾベンゼン基のパラマグネティックプローブを使用した.
タンパク質の周りの磁場分布が光によって変化する.
異なる探査状態に対応する異なる核磁気共振 (NMR) スペクトルを取得しました.

主要な成果:

単一のプローブから 2 つの独立した PCS セットを取得しました.
タンパク質の周りの磁場の光誘発による再分布を証明した.
新しい構造的制約の獲得を追加変異なしに可能にした.

結論:

アゾベンゼン探査機は構造研究のための多用途で効率的なツールです.
パラマグネティックセンターの動きに対する光駆動制御は,構造データを取得することを簡素化します.
このアプローチは,高度なNMRアプリケーションのためのテンソール交換可能なパラマグネットタグの開発を容易にする.

さらに関連する動画

Measuring Interactions of Globular and Filamentous Proteins by Nuclear Magnetic Resonance Spectroscopy NMR and Microscale Thermophoresis MST

Measuring Interactions of Globular and Filamentous Proteins by Nuclear Magnetic Resonance Spectroscopy NMR and Microscale Thermophoresis MST

Published on: November 2, 2018

Atomic Scale Structural Studies of Macromolecular Assemblies by Solid-state Nuclear Magnetic Resonance Spectroscopy

Atomic Scale Structural Studies of Macromolecular Assemblies by Solid-state Nuclear Magnetic Resonance Spectroscopy

Published on: September 17, 2017

関連する概念動画

Insensitive Nuclei Enhanced by Polarization Transfer (INEPT)

Insensitive Nuclei Enhanced by Polarization Transfer (INEPT)

Insensitive Nuclei Enhanced by Polarization Transfer (INEPT) is an advanced Nuclear Magnetic Resonance (NMR) technique specifically designed to detect and enhance the signals of low-abundance nuclei, such as carbon-13 and nitrogen-15, in small molecules. The fundamental principle behind INEPT is the transfer of polarization from a more abundant and highly polarizable nucleus, typically hydrogen-1, to the low-abundance nucleus of interest. This process effectively boosts the NMR signal of the...

NMR Spectroscopy: Chemical Shift Overview

NMR Spectroscopy: Chemical Shift Overview

The position of the absorption signal of a sample is reported relative to the position of the signal of tetramethylsilane (TMS), which is added as an internal reference while recording spectra. The difference between the absorption frequencies of the sample and TMS (in Hz) is divided by the spectrometer operating frequency (in MHz) to obtain a dimensionless quantity called the chemical shift. It is reported on the δ (delta) scale and expressed in parts per million.
For instance, the proton...

Chemical Shift: Internal References and Solvent Effects

Chemical Shift: Internal References and Solvent Effects

In an NMR sample, precise measurement of the absolute absorption frequencies of nuclei is difficult. A standard internal reference compound is added, and the frequency difference between the reference signal and sample signals is measured.
The internal reference compound generally used in NMR spectroscopy is tetramethylsilane (TMS). TMS is preferred because it is chemically inert, soluble in NMR solvents, and easily removable. Also, the highly shielded methyl protons in TMS yield an intense...

¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)

¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)

When proton-coupled carbon-13 spectra are simplified by a broadband proton decoupling technique, structural information about the coupled protons is lost. Distortionless enhancement by polarization transfer (DEPT) is a technique that provides information on the number of hydrogens attached to each carbon in a molecule. While the DEPT experiment utilizes complex pulse sequences, the pulse delay and flip angle are specifically manipulated. The resulting signals have different phases depending on...

Proton (¹H) NMR: Chemical Shift

Proton (¹H) NMR: Chemical Shift

Organic molecules primarily contain carbon and hydrogen atoms. While all the hydrogen isotopes are NMR-active, protium or hydrogen-1 is the most abundant. It has a significant energy separation between its nuclear spin states due to its large gyromagnetic ratio. As per Boltzmann's distribution, an increase in the energy separation implies a greater excess population of nuclei available for excitation, resulting in a strong NMR absorption signal.
Absorption signals of all the protium nuclei...

¹H NMR: Interpreting Distorted and Overlapping Signals

¹H NMR: Interpreting Distorted and Overlapping Signals

Spin systems where the difference in chemical shifts of the coupled nuclei is greater than ten times J are called first-order spin systems. These nuclei are weakly coupled, and their chemical shifts and coupling constant can generally be estimated from the well-separated signals in the spectrum.
As Δν decreases and the signals move closer, the doublets appear increasingly distorted. The intensities of the inner lines increase at the cost of those of the outer lines as the signals are...