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関連する概念動画

Amplifying Signals via Second Messengers01:15

Amplifying Signals via Second Messengers

8.4K
Many receptor binding ligands are hydrophilic; they do not cross the cell membrane but bind to cell-surface receptors. Thus, their message must be relayed by second messengers present in the cell cytoplasm. There are several second messenger pathways, each with its own way of relaying information. For example, the G protein-coupled receptors can activate both phosphoinositol and cyclic AMP (cAMP) second messenger pathways. The phosphoinositol pathway is active when the receptor induces...
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Signal Transduction: Overview01:26

Signal Transduction: Overview

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Cells respond to many types of information, often through receptor proteins positioned on the membrane. They respond to chemical signals, such as hormones, neurotransmitters, and other signaling molecules, initiating a series of molecular reactions to produce an appropriate response. This is called signal transduction. Cells also coordinate different responses elicited by the same signaling molecule via mediators, allowing molecular cross-talk.
Typically, signal transduction involves three...
11.4K
Single-pass Transmembrane Proteins01:25

Single-pass Transmembrane Proteins

6.5K
Integral membrane proteins are tightly associated with the cell membrane and play a crucial role in cell communication, signaling, adhesion, and transport of the molecules. Some integral membrane proteins are present only in the membrane monolayer. For example, the enzyme fatty acid amide hydrolase is present in the cytoplasmic side of the membrane monolayer. In contrast, another type of integral membrane protein, also known as a transmembrane protein, spans across the membrane. Transmembrane...
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Amplifying Signals via Enzymatic Cascade01:22

Amplifying Signals via Enzymatic Cascade

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When a ligand binds to a cell-surface receptor, the receptor's intracellular domain changes shape, which may either activate its enzyme function or allow its binding to other molecules. The initial signal is amplified by most signal transduction pathways. This means that a single ligand molecule can activate multiple molecules of a downstream target. Proteins that relay a signal are most commonly phosphorylated at one or more sites, activating or inactivating the protein. Kinases catalyze...
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Tagging and Fusion Proteins01:24

Tagging and Fusion Proteins

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Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...
8.3K
Conservation of Protein Domains Over Different Proteins02:26

Conservation of Protein Domains Over Different Proteins

14.0K
Protein domains are small structurally independent units that are part of a single amino acid chain.  Although these domains are often structurally independent, they may rely on synergistic effects to perform their functions as part of a larger protein. Protein domains may be conserved within the same organism, as well as across different organisms.
A limited set of protein domains often duplicate and recombine during evolution. These domains can be organized in different combinations to...
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Updated: Jan 15, 2026

Dissecting Multi-protein Signaling Complexes by Bimolecular Complementation Affinity Purification BiCAP
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Dissecting Multi-protein Signaling Complexes by Bimolecular Complementation Affinity Purification BiCAP

Published on: June 15, 2018

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多重性は,単一のタンパク質レベルで信号処理を可能にします.

Xiaoyu Wu1, Yuanqi Jia1, Tong Zhang2

  • 1School of Life Sciences, Westlake University, Hangzhou 310030, China.

Journal of the American Chemical Society
|October 15, 2025
PubMed
まとめ
この要約は機械生成です。

精密に細胞を標的にする 多価タンパク質結合剤を 設計する計算モデルを開発しました このツールは,選択的な細胞相互作用のための細胞表面抗原のアイデンティティと密度を感知できる結合物質の作成をガイドします.

さらに関連する動画

Simultaneous Affinity Enrichment of Two Post-Translational Modifications for Quantification and Site Localization
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Simultaneous Affinity Enrichment of Two Post-Translational Modifications for Quantification and Site Localization

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Proteome-wide Quantification of Labeling Homogeneity at the Single Molecule Level
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Proteome-wide Quantification of Labeling Homogeneity at the Single Molecule Level

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関連する実験動画

Last Updated: Jan 15, 2026

Dissecting Multi-protein Signaling Complexes by Bimolecular Complementation Affinity Purification BiCAP
06:45

Dissecting Multi-protein Signaling Complexes by Bimolecular Complementation Affinity Purification BiCAP

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Simultaneous Affinity Enrichment of Two Post-Translational Modifications for Quantification and Site Localization
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Proteome-wide Quantification of Labeling Homogeneity at the Single Molecule Level
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Proteome-wide Quantification of Labeling Homogeneity at the Single Molecule Level

Published on: April 19, 2019

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科学分野:

  • バイオテクノロジー
  • 分子工学
  • 合成生物学

背景:

  • 細胞における合成信号処理は,複雑な分子ネットワークや,より単純な単一分子メカニズムを利用することができる.
  • 単一分子プロセッサは,遺伝的負荷を軽減し,より簡単な配送を提供し,多価タンパク質結合剤は,細胞抗原プロファイルに基づいてユニークな行動を示します.

研究 の 目的:

  • 細胞表面での多価相互作用を設計するための定量的な方法の欠如に対処する.
  • 細胞表面結合と信号処理のための多価タンパク質の設計を導くための計算ツールを開発する.

主な方法:

  • 多価抗原感知シミュレータ (MASS) 計算モデルの開発.
  • MASSモデルの検証 in vitroおよび細胞結合実験

主要な成果:

  • MASSモデルは,細胞表面抗原のアイデンティティを感知し,選択的に細胞を殺すための多価結合剤の設計を正確に予測しました.
  • 実験的検証は,抗原密度感知におけるバレンシーとモノバレンスの非単調的な関係を捉えるモデルの能力を確認した.
  • このモデルは,抗原のアイデンティティと密度を同時に感知するバインダーを設計する際の予測精度を実証した.

結論:

  • 開発されたMASSモデルは,特定の細胞感知能力を持つ多価タンパク質を設計するための実用的なガイドラインを提供します.
  • シミュレータは,様々な用途の多価タンパク質結合剤の迅速かつ正確なシリコンスクリーニングを可能にします.