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関連する概念動画

Ribosome Profiling02:24

Ribosome Profiling

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Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
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Regulation of Expression at Multiple Steps01:23

Regulation of Expression at Multiple Steps

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The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...
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Transfer RNA Synthesis02:36

Transfer RNA Synthesis

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One of the unique features of tRNA is the presence of modified bases. In some tRNAs, modified bases account for nearly 20% of the total bases in the molecule. Altogether, these unusual bases protect the tRNA from enzymatic degradation by RNases.
Each of these chemical modifications is carried by a specific enzyme, post-transcription. All of these enzymes have unique base and site-specificity. Methylation, the most common chemical modification, is carried by at least nine different enzymes, with...
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RNA Editing02:23

RNA Editing

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RNA editing is a post-transcriptional modification where a precursor mRNA (pre-mRNA) nucleotide sequence is changed by base insertion, deletion, or modification. The extent of RNA editing varies from a few hundred bases, in mitochondrial DNA of trypanosomes, to a just single base, in nuclear genes of mammals. Even a single base change in the pre-mRNA can convert a codon for one amino acid into the codon for another amino acid or a stop codon. This type of re-coding can significantly affect the...
9.8K
Translational Regulation01:29

Translational Regulation

531
Translational regulation in prokaryotes ensures efficient protein synthesis by controlling ribosome access to mRNA. This regulation is mediated by secondary RNA structures, including translational riboswitches, RNA thermometers, and small RNAs (sRNAs), which respond to intracellular and environmental signals to modulate gene expression.Translational RiboswitchesRiboswitches in the leader region of mRNAs can regulate translation by altering the accessibility of the Shine-Dalgarno (SD) sequence,...
531
Riboswitches01:56

Riboswitches

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Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
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関連する実験動画

Updated: Jan 15, 2026

Quantitative Immunofluorescence to Measure Global Localized Translation
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Quantitative Immunofluorescence to Measure Global Localized Translation

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メッセージリボ核酸翻訳に対する改変のサイト固有の効果を明らかにするために精密に改変されたリボ核酸の合成

Yufan Pan1, Chenyou Zhu1, Yuan Zhuang1,2

  • 1Engineering Research Center of Advanced Rare Earth Materials (Ministry of Education), Department of Chemistry, Tsinghua University, Beijing, 100084, China.

Journal of the American Chemical Society
|October 16, 2025
PubMed
まとめ

この研究は,サイト特異的に改変された長い単一鎖RNA (ssRNA) の作成のための新しい方法を導入しています. この進歩はRNAの改変を正確に制御し,mRNA治療の安全性と有効性を高めます.

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A Method for Measuring RNA N6-methyladenosine Modifications in Cells and Tissues
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A Method for Measuring RNA N6-methyladenosine Modifications in Cells and Tissues

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A Rapid High-throughput Method for Mapping Ribonucleoproteins RNPs on Human pre-mRNA
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A Rapid High-throughput Method for Mapping Ribonucleoproteins RNPs on Human pre-mRNA

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関連する実験動画

Last Updated: Jan 15, 2026

Quantitative Immunofluorescence to Measure Global Localized Translation
09:13

Quantitative Immunofluorescence to Measure Global Localized Translation

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A Method for Measuring RNA N6-methyladenosine Modifications in Cells and Tissues
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科学分野:

  • 生物化学
  • 分子生物学
  • 薬物開発

背景:

  • RNAの改変はmRNA治療の開発に不可欠です.
  • 場所特有の改変の精度と生物安全性に関する懸念がある.
  • 正確な改変効果を理解することは,mRNA治療の進歩に不可欠です.

研究 の 目的:

  • サイト特異的に改変された長いssRNAを合成するためのテンプレート指向の戦略を開発する.
  • 正確に修正されたmRNAパターンが翻訳に与える影響を調査する.
  • 次世代の治療法とRNAの表遺伝学におけるmRNA改変のためのガイドラインを提供すること.

主な方法:

  • 長ssRNA (300nt以上) のテンプレート指向合成戦略を開発した.
  • m6A,m5C,m1Ψ, Ψ,I,Br-dU,Cy3,Cy5,FAM,2'-F,2'-OMe,2'-MOE,2'-Propargyl,LNA,cET,PSを含む単基解像度修正を達成した.
  • 特定の修飾パターンがmRNA翻訳に与える機能的影響を調査した.

主要な成果:

  • シングルベース解像度でサイト固有の改変で長いssRNAを合成しました.
  • 特定のサイトでの m1Ψ 改変が翻訳の精度を高めることを示した.
  • m1Ψの改変は低免疫性を持ちながら,翻訳を改善することが観察された.

結論:

  • 開発された戦略は,長いssRNAのRNA改変を正確に制御することを可能にします.
  • サイト固有のm1Ψ改変は,mRNA翻訳の精度と安全性を改善するための有望なアプローチを提供します.
  • この研究は,先進的なmRNA薬を開発し,RNAの表遺伝学を進めるための基盤を提供します.