関連する実験動画

Updated: Aug 4, 2026

DNA Polymerase Activity Assay Using Near-infrared Fluorescent Labeled DNA Visualized by Acrylamide Gel Electrophoresis
07:38

DNA Polymerase Activity Assay Using Near-infrared Fluorescent Labeled DNA Visualized by Acrylamide Gel Electrophoresis

Published on: October 6, 2017

母乳のDNAポリメラーゼ活動

B I Gerwin, P S Ebert, H C Chopra

    Science (New York, N.Y.)
    |April 15, 1973
    PubMed
    まとめ

    人間の牛乳には,ユニークな性質を持つDNAポリメラーゼが含まれています. ある酵素は,テンプレート偏好でRNA腫瘍ウイルス酵素に似ていますが,染色体の行動は異なっており,その活動は癌の家族歴と相関していません.

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    Replication in Eukaryotes

    Overview
    Proofreading01:43

    Proofreading

    Synthesis of new DNA molecules starts when DNA polymerase links nucleotides together in a sequence that is complementary to the template DNA strand. DNA polymerase has a higher affinity for the correct base to ensure fidelity in DNA replication. The DNA polymerase furthermore proofreads during replication, using an exonuclease domain that cuts off incorrect nucleotides from the nascent DNA strand.Errors during Replication Are Corrected by the DNA Polymerase EnzymeGenomic DNA is synthesized in...
    PCR01:32

    PCR

    Overview
    Bacterial RNA Polymerase00:43

    Bacterial RNA Polymerase

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    In most genes, the transcription site is a single base present upstream of the coding sequence. Though RNAP is a catalytically efficient enzyme, it does not recognize...
    Proofreading01:31

    Proofreading

    Synthesis of new DNA molecules is carried out by the enzyme DNA polymerase, which adds nucleotides on the daughter strand complementary to the template DNA strand. DNA polymerase has a higher affinity to add the correct base and ensures fidelity during DNA replication. Furthermore,  it exhibits proofreading activity during replication, using an exonuclease domain that cuts off incorrect nucleotides from the nascent DNA strand.
    Errors During Replication are Corrected by the DNA Polymerase Enzyme
    Replication in Eukaryotes01:29

    Replication in Eukaryotes

    In eukaryotic cells, DNA replication is highly conserved and tightly regulated. Multiple linear chromosomes must be duplicated with high fidelity before cell division, so there are many proteins that fulfill specialized roles in the replication process. Replication occurs in three phases: initiation, elongation, and termination, and ends with two complete sets of chromosomes in the nucleus.
    Many Proteins Orchestrate Replication at the Origin
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