関連する実験動画

Updated: Aug 18, 2026

Isolation of Fidelity Variants of RNA Viruses and Characterization of Virus Mutation Frequency
18:10

Isolation of Fidelity Variants of RNA Viruses and Characterization of Virus Mutation Frequency

Published on: June 16, 2011

哺乳類RNA腫瘍ウイルスの分子関連性は,DNAハイブリッド化によって決定される

D K Haapala, P J Fischinger

    Science (New York, N.Y.)
    |June 1, 1973
    PubMed
    まとめ

    研究者らは,DNA-RNAのハイブリッド化により,猫およびネズミの白血病ウイルスを含む6種類の哺乳類C型ウイルスを区別しました. この方法により,これらのレトロウイルス間の核酸塩基配列の有意な多様性が明らかになり,それらの分類に役立ちました.

    さらに関連する動画

    Detection of Viral RNA by Fluorescence in situ Hybridization (FISH)10:16

    Detection of Viral RNA by Fluorescence in situ Hybridization (FISH)

    Published on: May 5, 2012

    Detection of the Genome and Transcripts of a Persistent DNA Virus in Neuronal Tissues by Fluorescent In situ Hybridization Combined with Immunostaining13:22

    Detection of the Genome and Transcripts of a Persistent DNA Virus in Neuronal Tissues by Fluorescent In situ Hybridization Combined with Immunostaining

    Published on: January 23, 2014

    関連する概念動画

    Viral Mutations00:36

    Viral Mutations

    A mutation is a change in the sequence of bases of DNA or RNA in a genome. Some mutations occur during replication of the genome due to errors made by the polymerase enzymes that replicate DNA or RNA. Unlike DNA polymerase, RNA polymerase is prone to errors because it is not capable of “proofreading” its work. Viruses with RNA-based genomes, like HIV, therefore accrue mutations faster than viruses with DNA-based genomes. Because mutation and recombination provide the raw material for adaptive...
    Evolutionary Relationships through Genome Comparisons02:54

    Evolutionary Relationships through Genome Comparisons

    Genome comparison is one of the excellent ways to interpret the evolutionary relationships between organisms. The basic principle of genome comparison is that if two species share a common feature, it is likely encoded by the DNA sequence conserved between both species. The advent of genome sequencing technologies in the late 20th century enabled scientists to understand the concept of conservation of domains between species and helped them to deduce evolutionary relationships across diverse...
    Southern Blot02:57

    Southern Blot

    Agarose gel electrophoresis is very useful in separating DNA fragments by size. Running a DNA ladder containing fragments of the known length alongside the sample helps determine the approximate length of the sample DNA fragments. However, additional steps are needed to verify the sequence identity of the sample DNA fragments.
    Denatured DNA fragments must be transferred onto a carrier membrane from the gel to make it accessible to a probe - a small ssDNA fragment complementary to the target DNA...
    In-situ Hybridization02:31

    In-situ Hybridization

    In situ hybridization (ISH) is a technique used to detect and localize specific DNA or RNA molecules in cells, tissue, or tissue sections using a labeled probe. The technique was first used in 1969 for the investigation of nucleic acids. It is currently an essential tool in scientific research and clinical settings, especially for diagnostic purposes.
    Types of probes and labels
    A probe is a complementary strand of DNA or RNA that binds to corresponding nucleotide sequences in a cell. Many...
    Rous Sarcoma Virus (RSV) and Cancer01:03

    Rous Sarcoma Virus (RSV) and Cancer

    Rous Sarcoma virus or RSV was discovered by F. Peyton Rous in the year 1911 as a filterable transmissible agent that could cause tumors in chickens. He won a Nobel Prize for this discovery in 1966. His experiments clearly demonstrated that some cancers could be caused by infectious agents and led to the discovery of many more cancer-causing viruses in animals as well as humans.
    RSV is a retrovirus that contains two copies of a plus-strand  RNA genome. Its genome consists of four main open...
    Modern Molecular Taxonomy01:29

    Modern Molecular Taxonomy

    Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...