Whole genome sequencing as a reliable alternative for Salmonella serotyping: a comparative study with the
Irene Mileto1,2, Greta Romano1, Stefano Gaiarsa1
1Department of Microbiology and Virology, Fondazione IRCCS Policlinico San Matteo, Pavia, Italy.
Frontiers in microbiology
|December 12, 2025
まとめ
Whole genome sequencing (WGS) offers a reliable method for classifying Salmonella enterica serotypes, outperforming traditional methods. This approach accurately identifies serotypes and can resolve cases where gold-standard methods fail, improving foodborne pathogen surveillance.
科学分野:
- Microbiology; Genomics; Food Safety
背景:
- Salmonella enterica is a significant foodborne pathogen causing global health concerns.; Serotyping based on O and H antigens is crucial for Salmonella classification but is laborious.; Traditional methods face challenges in speed and accuracy for classifying over 2600 Salmonella serotypes.
研究 の 目的:
- To evaluate whole genome sequencing (WGS) as a method for Salmonella enterica classification.; To compare WGS-based serotyping with the gold-standard serum agglutination method.; To assess the efficiency and accuracy of WGS for identifying Salmonella serotypes.
主な方法:
- Collected 282 Salmonella enterica isolates from January 2021 to December 2024.; Performed gold-standard serotyping using O and H antigens via serum agglutination.; Conducted whole genome sequencing (WGS) using Illumina MiSeq and analyzed reads with SeqSero2 and SISTR tools.
主要な成果:
- WGS accurately predicted serotypes for 236 out of 282 isolates, showing high concordance with the gold-standard method.; Genomic typing successfully identified 34 isolates where the gold-standard method failed.; WGS classified all isolates, maintaining consistency with existing Salmonella subspecies and serovar classifications.
結論:
- Whole genome sequencing is a robust and reliable method for Salmonella typing.; WGS offers advantages in speed and sample throughput compared to traditional methods.; WGS enhances identification capabilities, successfully typing isolates that are challenging for gold-standard methods.
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