ホースラディッシュペルオキシダーゼを用いた細胞表面タンパク質の同定プロトコル

関連する実験動画

Updated: Jan 8, 2026

Quantitation of Protein Expression and Co-localization Using Multiplexed Immuno-histochemical Staining and Multispectral Imaging

Quantitation of Protein Expression and Co-localization Using Multiplexed Immuno-histochemical Staining and Multispectral Imaging

Published on: April 8, 2016

Ling Wu¹, Vijaya Pandey², James A Wohlschlegel²

¹Department of Physiology, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA, USA.

|December 12, 2025

Abstract:

Cell-surface proteins (CSPs) play a central role in intercellular communication. Here, we outline a protocol for the cell-type-specific proteomic profiling of CSPs in mice based on horseradish peroxidase (HRP). We describe steps for adeno-associated virus (AAV) injection, biotinylation reaction, tissue homogenization, streptavidin pull-down, and mass spectrometry characterization, followed by quality control and data interpretation. This approach enables systematic identification of CSPs underlying cell-cell interactions in health and disease. For complete details on the use and execution of this protocol, please refer to Wu et al.¹.

キーワード:

質量分析分子生物学分子/化学プローブ神経科学タンパク質生化学プロテオミクス

さらに関連する動画

Assaying Proteasomal Degradation in a Cell-free System in Plants

Assaying Proteasomal Degradation in a Cell-free System in Plants

Published on: March 26, 2014

Detection of In Situ Protein-protein Complexes at the Drosophila Larval Neuromuscular Junction Using Proximity Ligation Assay

Detection of In Situ Protein-protein Complexes at the Drosophila Larval Neuromuscular Junction Using Proximity Ligation Assay

Published on: January 20, 2015

関連する概念動画

Western Blotting

Western Blotting

Western blotting is an analytical technique for protein identification. It has various applications in immunology and medicine, including detecting diseases like bovine spongiform encephalopathy, mad cow disease, and human and feline immunodeficiency virus from biological samples.
The technique begins with separating proteins from the sample using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), followed by protein transfer, immunoblotting, and finally, protein detection.

Labeling DNA Probes

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...